biostudies-literatureAkinosho ANIAMS NIH HHSNIH HHSRP107034https://www.ebi.ac.uk/biostudies/studies/S-EPMC12604853biostudies-literatureUnknown14Previously, it was shown that learned avoidance of <i>Pseudomonas aeruginosa</i> PA14 in <i>Caenorhabditis elegans</i> can be transmitted to untrained offspring (Kaletsky et al., 2025). Here, we show an independent validation of this phenomenon under the originally specified assay conditions. Adapting the Murphy lab protocol, worms trained on PA14 develop significant avoidance that persists in F1 and F2 generations, with reduced magnitude after P0. These results align with the Murphy group's findings and address a recent report from the Hunter group that did not detect persistence beyond F1.eLifeIndependent validation of transgenerational inheritance of learned pathogen avoidance in &lt;i&gt;Caenorhabditis elegans&lt;/i&gt;.PMC126048532R15AR068583-02P40 OD010440R15 AR068583Akinosho AFloyd KAlexander JVidal-Gadea AGfalseIndependent validation of transgenerational inheritance of learned pathogen avoidance in &lt;i&gt;Caenorhabditis elegans&lt;/i&gt;.Previously, it was shown that learned avoidance of <i>Pseudomonas aeruginosa</i> PA14 in <i>Caenorhabditis elegans</i> can be transmitted to untrained offspring (Kaletsky et al., 2025). Here, we show an independent validation of this phenomenon under the originally specified assay conditions. Adapting the Murphy lab protocol, worms trained on PA14 develop significant avoidance that persists in F1 and F2 generations, with reduced magnitude after P0. These results align with the Murphy group's findings and address a recent report from the Hunter group that did not detect persistence beyond F1.2025-01-01T00:00:00Z2025 Nov2026-06-05T15:11:26.012Z2026-05-18T03:13:05.243ZS-EPMC126048534121781210.7554/eLife.107034