{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["He W"],"funding":["General Program of National Natural Science Foundation of China","Natural science foundation of Science and Technology Commission of Shanghai Municipality","National Natural Science Foundation of China","National Key Research and Development Program of China"],"pagination":["384"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12606830"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["26(1)"],"pubmed_abstract":["High-throughput sequencing with chemical labeling enables robust, transcriptome-wide detection of RNA modifications at single-nucleotide resolution. However, these methods typically require substantial RNA amounts due to harsh treatments. We introduce Uli-epic, an innovative library construction strategy that enables profiling epitranscriptomic modifications using 100 pg to 1 ng of RNA. Utilizing Uli-epic BID-seq, we investigate pseudouridine (Ψ) sites in neural stem cells and sperm RNA from wild-type and fetal growth restriction mice, using only 500 pg of rRNA-depleted RNA. Uli-epic GLORI quantifies m<sup>6</sup>A in sperm and neural stem cells from wild-type and fetal growth restriction mice, using 10 ng of rRNA-depleted RNA."],"journal":["Genome biology"],"pubmed_title":["Uli-epic: profiling RNA modifications from ultra-low input samples."],"pmcid":["PMC12606830"],"funding_grant_id":["21ZR1480300","82230054","32471340","2021YFA1100400","82271723"],"pubmed_authors":["Peng Y","He W","Jiang X","Chen W","Xu C","Chang S","Zhu W","Hu L","Wang Y","Kang J"],"additional_accession":[]},"is_claimable":false,"name":"Uli-epic: profiling RNA modifications from ultra-low input samples.","description":"High-throughput sequencing with chemical labeling enables robust, transcriptome-wide detection of RNA modifications at single-nucleotide resolution. However, these methods typically require substantial RNA amounts due to harsh treatments. We introduce Uli-epic, an innovative library construction strategy that enables profiling epitranscriptomic modifications using 100 pg to 1 ng of RNA. Utilizing Uli-epic BID-seq, we investigate pseudouridine (Ψ) sites in neural stem cells and sperm RNA from wild-type and fetal growth restriction mice, using only 500 pg of rRNA-depleted RNA. Uli-epic GLORI quantifies m<sup>6</sup>A in sperm and neural stem cells from wild-type and fetal growth restriction mice, using 10 ng of rRNA-depleted RNA.","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Nov","modification":"2026-06-05T13:40:22.749Z","creation":"2026-05-17T03:12:26.834Z"},"accession":"S-EPMC12606830","cross_references":{"pubmed":["41219823"],"doi":["10.1186/s13059-025-03857-3"]}}