biostudies-literatureRios-Delgado GNational Institute of Allergy and Infectious DiseasesAgence Nationale de la RechercheU.S. Department of AgricultureNIAID NIH HHSNational Science Foundatione0141525https://www.ebi.ac.uk/biostudies/studies/S-EPMC12607789biostudies-literatureUnknown16(11)Bacterial regulatory RNAs (sRNAs) are commonly short non-coding RNAs that function as pleiotropic regulators by post-transcriptionally impacting mRNA stability and/or translation. They play significant roles in bacterial physiology and are typically expressed in response to specific environmental stimuli such as nutrient limitation. The bacterial pathogen <i>Staphylococcus aureus</i> faces decreased access to essential metal ions, including iron, in the mammalian host via a process called nutritional immunity. In response to host-mediated iron limitation, <i>S. aureus</i> expresses the sRNA IsrR, which coordinates an iron-sparing response by downregulating the expression of mRNAs coding for iron-requiring proteins or processes. Herein, we utilized MS2-Affinity Purification coupled with RNAmBioThe iron-regulated small regulatory RNA IsrR modulates expression of genes utilized for dioxygen metabolism and heme synthesis in &lt;i&gt;Staphylococcus aureus&lt;/i&gt;.PMC12607789R01 AI139100R01 AI155611ANR-20-CE12-0021ANR-10-IDEX-0002AI1556111750624ANR-17-EURE-0023ANR-20-SFRI-0012NE-2248Lalaouna DKehl-Fie TRios-Delgado GBoyd JMMcFarlane RKim JZheng VParker DfalseThe iron-regulated small regulatory RNA IsrR modulates expression of genes utilized for dioxygen metabolism and heme synthesis in &lt;i&gt;Staphylococcus aureus&lt;/i&gt;.Bacterial regulatory RNAs (sRNAs) are commonly short non-coding RNAs that function as pleiotropic regulators by post-transcriptionally impacting mRNA stability and/or translation. They play significant roles in bacterial physiology and are typically expressed in response to specific environmental stimuli such as nutrient limitation. The bacterial pathogen <i>Staphylococcus aureus</i> faces decreased access to essential metal ions, including iron, in the mammalian host via a process called nutritional immunity. In response to host-mediated iron limitation, <i>S. aureus</i> expresses the sRNA IsrR, which coordinates an iron-sparing response by downregulating the expression of mRNAs coding for iron-requiring proteins or processes. Herein, we utilized MS2-Affinity Purification coupled with RNA2025-01-01T00:00:00Z2025 Nov2026-06-05T13:52:07.655Z2026-05-17T03:13:02.351ZS-EPMC126077894104206110.1128/mbio.01415-25