biostudies-literatureUnknown63(11)Pretot EAmoebiasis is a cosmopolitan parasitic disease caused by <i>Entamoeba histolytica</i>. The most lethal form is extra-intestinal amoebiasis, mainly manifesting as liver abscesses. Diagnosis is based on clinical, radiological, and biological tests. However, there are currently few serology reagents still available on the European market for <i>in vitro</i> diagnosis, and comparative studies of existing reagents are required. We evaluated the performances of the four currently available reagents, either as standalone tests or in combination. Two enzyme-linked immunosorbent assays (ELISAs) for the detection of <i>E. histolytica</i> IgG were assessed: one manufactured by Bordier and the other by NovaTec. Additionally, an indirect hemagglutination technique, ELI.H.A <i>Amoeba</i>, and a latex paJournal of clinical microbiologye0017925https://www.ebi.ac.uk/biostudies/studies/S-EPMC12607903biostudies-literatureSerodiagnosis of amoebic abscess: a retrospective diagnostic accuracy study of kits marketed in Europe.PMC12607903Moreno-Sabater AGabriel FGrenouillet FTouafek FDelcey LDupont DPretot ESokol HBrenier-Pinchart M-PAmiot CFricker-Hidalgo HMarteau ATirard-Collet PfalseSerodiagnosis of amoebic abscess: a retrospective diagnostic accuracy study of kits marketed in Europe.Amoebiasis is a cosmopolitan parasitic disease caused by <i>Entamoeba histolytica</i>. The most lethal form is extra-intestinal amoebiasis, mainly manifesting as liver abscesses. Diagnosis is based on clinical, radiological, and biological tests. However, there are currently few serology reagents still available on the European market for <i>in vitro</i> diagnosis, and comparative studies of existing reagents are required. We evaluated the performances of the four currently available reagents, either as standalone tests or in combination. Two enzyme-linked immunosorbent assays (ELISAs) for the detection of <i>E. histolytica</i> IgG were assessed: one manufactured by Bordier and the other by NovaTec. Additionally, an indirect hemagglutination technique, ELI.H.A <i>Amoeba</i>, and a latex pa2025-01-01T00:00:00Z2025 Nov2026-06-05T13:23:36.779Z2026-06-05T03:06:01.165ZS-EPMC126079034111754510.1128/jcm.00179-25