{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Dong X"],"funding":["National Key R ＆ D Program of China","Project of the Seed Industry Revitalization of Department of Agriculture and Rural Affairs of Guangdong Province","Open Projects of Key Laboratory for Poultry Genetics and Breeding of Jiangsu Province","Guangdong Basic and Applied Basic Research Foundation","Biological Breeding-National Science and Technology Major Project"],"pagination":["3076"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12607967"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["15(21)"],"pubmed_abstract":["Hyperpigmentation of the visceral peritoneum (HVP) is a pigmentation abnormality in chickens that adversely affects carcass appearance, consumer acceptance, and poultry production. However, the genetic basis of HVP remains unclear. To investigate the causes and regulatory mechanisms of HVP, we employed high-performance liquid chromatography (HPLC), bulk RNA sequencing (RNA-seq), qRT-PCR, Western blotting, and siRNA interference. Additionally, single-cell RNA sequencing (scRNA-seq) was used to examine gene expression at the cellular level. Anatomical examination and hematoxylin and eosin (HE) staining revealed melanin deposition in the peritoneum of HVP-affected chickens. Spectrophotometric analysis at 500 nm showed significantly higher absorbance in the HVP group (<i>p</i> < 0.05), which c"],"journal":["Animals : an open access journal from MDPI"],"pubmed_title":["Visceral Peritoneum Hyperpigmentation in Chickens Is Associated with &lt;i&gt;DCT&lt;/i&gt; Expression."],"pmcid":["PMC12607967"],"funding_grant_id":["2022B1515120049","2023ZD04064","JQLAB-KF-202201","2021YFD1300100","2022-XPY-05-001"],"pubmed_authors":["Xing J","Li Z","Dong X","Nie Q","Shu J","Liu Q","Mo G","Li M"],"additional_accession":[]},"is_claimable":false,"name":"Visceral Peritoneum Hyperpigmentation in Chickens Is Associated with &lt;i&gt;DCT&lt;/i&gt; Expression.","description":"Hyperpigmentation of the visceral peritoneum (HVP) is a pigmentation abnormality in chickens that adversely affects carcass appearance, consumer acceptance, and poultry production. However, the genetic basis of HVP remains unclear. To investigate the causes and regulatory mechanisms of HVP, we employed high-performance liquid chromatography (HPLC), bulk RNA sequencing (RNA-seq), qRT-PCR, Western blotting, and siRNA interference. Additionally, single-cell RNA sequencing (scRNA-seq) was used to examine gene expression at the cellular level. Anatomical examination and hematoxylin and eosin (HE) staining revealed melanin deposition in the peritoneum of HVP-affected chickens. Spectrophotometric analysis at 500 nm showed significantly higher absorbance in the HVP group (<i>p</i> < 0.05), which c","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Oct","modification":"2026-05-18T03:18:09.045Z","creation":"2026-05-18T03:12:50.945Z"},"accession":"S-EPMC12607967","cross_references":{"pubmed":["41227407"],"doi":["10.3390/ani15213076"]}}