biostudies-literatureLeng YJthe Priority Academic Program Development of Jiangsu Higher Education Institutionsthe open funds of the Jiangsu Key Laboratory of Crop Genomics and Molecular BreedingJiangsu Province Governmentthe Natural Science Foundation of the Jiangsu Higher Education Institutions of China3373https://www.ebi.ac.uk/biostudies/studies/S-EPMC12608271biostudies-literatureUnknown14(21)Leaves represent an important organ in plant photosynthesis, and moderately rolled leaves would be beneficial in establishing an ideal plant architecture and thereby increasing rice yields. In this study, a stable inherited rolled leaf mutant was obtained via ethyl methanesulfonate (EMS) mutagenesis from <i>japonica</i> variety WYJ27, which was named <i>rll2</i> (<i>rolling leaf 2</i>). <i>rll2</i> showed a leaf-rolling phenotype at the seedling stage, which increased with growth. Compared with the wild type, the leaves at all levels of <i>rll2</i> were significantly shorter and narrower, and the leaf-rolling index gradually decreased from the highest leaf to the third-highest leaf. Semi-thin sections showed that the bulliform cells of <i>rll2</i> were significantly larger than those of thPlants (Basel, Switzerland)Rolling Leaf 2 Controls Leaf Rolling by Regulating Adaxial-Side Bulliform Cell Number and Size in Rice.PMC12608271No22KJA210003BE2022336PL202403Gao JPYang ZDJin SKWei XZhang MQLeng YJLiu HBYang QQCai XLHuan HDZhou WYQiang SYZheng YFLu SXu FXZhang HCTao TCui WXfalseRolling Leaf 2 Controls Leaf Rolling by Regulating Adaxial-Side Bulliform Cell Number and Size in Rice.Leaves represent an important organ in plant photosynthesis, and moderately rolled leaves would be beneficial in establishing an ideal plant architecture and thereby increasing rice yields. In this study, a stable inherited rolled leaf mutant was obtained via ethyl methanesulfonate (EMS) mutagenesis from <i>japonica</i> variety WYJ27, which was named <i>rll2</i> (<i>rolling leaf 2</i>). <i>rll2</i> showed a leaf-rolling phenotype at the seedling stage, which increased with growth. Compared with the wild type, the leaves at all levels of <i>rll2</i> were significantly shorter and narrower, and the leaf-rolling index gradually decreased from the highest leaf to the third-highest leaf. Semi-thin sections showed that the bulliform cells of <i>rll2</i> were significantly larger than those of th2025-01-01T00:00:00Z2025 Nov2026-05-17T03:21:49.046Z2026-05-17T03:13:09.243ZS-EPMC126082714122592310.3390/plants14213373