{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Baker D"],"funding":["Bundesministerium f??r Bildung und Forschung"],"pagination":["24295-24305"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12613151"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["97(44)"],"pubmed_abstract":["This study introduces a clinical lipidomics platform leveraging fragment-based quantification on parallel reaction monitoring (PRM)-parallel accumulation serial fragmentation (PASEF) for lipid quantification. An isomeric model, termed \"SN regression model\", built on specific PASEF-fragment ion patterns, was developed for the quantification of coeluting sn positional isomers without prior derivatization. This PASEF-isomeric lipidomics aids in the resolution and quantification of 176 lipid isomers coeluting in chromatography and/or ion mobility dimensions, expanding the lipidome quantitative coverage to 481 plasma lipids covering 14 lipid subclasses with CV <40% for 32 plasma replicates. We demonstrated the method's advantage for clinical research by detailed quantitative lipidomic phenotypi"],"journal":["Analytical chemistry"],"pubmed_title":["prm-PASEF-Based Quantification and Isomeric Model for Extended Coverage of Human Plasma Lipidome in Parkinson's Disease."],"pmcid":["PMC12613151"],"funding_grant_id":["16LK0241K","031 L0217 A","03ZU1202EB"],"pubmed_authors":["Gonzalez Escamilla G","Bindila L","Groppa S","Janitschke D","Devaux Y","Baker D","Schroter N"],"additional_accession":[]},"is_claimable":false,"name":"prm-PASEF-Based Quantification and Isomeric Model for Extended Coverage of Human Plasma Lipidome in Parkinson's Disease.","description":"This study introduces a clinical lipidomics platform leveraging fragment-based quantification on parallel reaction monitoring (PRM)-parallel accumulation serial fragmentation (PASEF) for lipid quantification. An isomeric model, termed \"SN regression model\", built on specific PASEF-fragment ion patterns, was developed for the quantification of coeluting sn positional isomers without prior derivatization. This PASEF-isomeric lipidomics aids in the resolution and quantification of 176 lipid isomers coeluting in chromatography and/or ion mobility dimensions, expanding the lipidome quantitative coverage to 481 plasma lipids covering 14 lipid subclasses with CV <40% for 32 plasma replicates. We demonstrated the method's advantage for clinical research by detailed quantitative lipidomic phenotypi","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Nov","modification":"2026-06-05T13:40:41.537Z","creation":"2026-05-17T03:12:36.628Z"},"accession":"S-EPMC12613151","cross_references":{"pubmed":["41143626"],"doi":["10.1021/acs.analchem.5c02340"]}}