biostudies-literatureHuang XMajor Science and Technology Projects of the Scientific and Technological Innovation PlatformUndergraduate Innovation and Entrepreneurship Training ProgramOpen Project of Key Lab. of Provincial Key LaboratoryAnqing Normal University Graduate Student Industry-University Collaborative Practice Program Special Fund GrantUniversity Research Projects of Anhui ProvincialMinistry of Education's 2024 Themed Case Study ProjectIndustry Academia-Research Project of Demonstration Research on Fungal Genome Analysis and Technological Innovation in Microbial Agricultural Wastewater TreatmentShenzhen Science and Technology Program737https://www.ebi.ac.uk/biostudies/studies/S-EPMC12613345biostudies-literatureUnknown25(1)A novel Gram-stain-negative, facultatively anaerobic, endospore-forming bacterium, designated DYY-L-2^T, was isolated from the saliva of a 69-year-old patient with chronic periodontitis in Hengyang, China. Phylogenetic analysis based on the 16S rRNA gene sequences revealed that strain DYY-L-2^T belonged to the genus Paenibacillus, with the highest similarity to Paenibacillus konsidensis LBY^T (98.6%), followed by Paenibacillus vini LAM0504^T (97.2%). Whole-genome sequencing yielded a complete circular chromosome of 5,642,305 bp with a genomic DNA G + C content of 50.8%. Overall genome relatedness index analysis indicated low average nucleotide identity (ANI < 80%) and in silico DNA-DNA hybridization (isDDH < 25%) values between strain DYY-L-2<sup>T</BMC microbiologyPhenotypic and genomic characterization of Paenibacillus salivarius sp. nov., isolated from the human oral cavity.PMC126133452024AH051076ZT-2410372012202305A12020022202410372004SH20250225(Industry-University Co-constructed Demonstration Course: Restoration Ecology, X2025xqkc007)202410372016Wxn202403JCYJ20230808105208017Xu LPeng YZhou QChen MHuang XMu DLing ZWu YCai XHuang PfalsePhenotypic and genomic characterization of Paenibacillus salivarius sp. nov., isolated from the human oral cavity.A novel Gram-stain-negative, facultatively anaerobic, endospore-forming bacterium, designated DYY-L-2^T, was isolated from the saliva of a 69-year-old patient with chronic periodontitis in Hengyang, China. Phylogenetic analysis based on the 16S rRNA gene sequences revealed that strain DYY-L-2^T belonged to the genus Paenibacillus, with the highest similarity to Paenibacillus konsidensis LBY^T (98.6%), followed by Paenibacillus vini LAM0504^T (97.2%). Whole-genome sequencing yielded a complete circular chromosome of 5,642,305 bp with a genomic DNA G + C content of 50.8%. Overall genome relatedness index analysis indicated low average nucleotide identity (ANI < 80%) and in silico DNA-DNA hybridization (isDDH < 25%) values between strain DYY-L-2<sup>T</2025-01-01T00:00:00Z2025 Nov2026-06-05T13:42:36.483Z2026-05-17T03:12:33.791ZS-EPMC126133454122534810.1186/s12866-025-04522-2