biostudies-literatureHo CTAmerican Heart AssociationUniversity of North CarolinaNCI NIH HHSNIGMS NIH HHSNIH HHSe202503068https://www.ebi.ac.uk/biostudies/studies/S-EPMC12614643biostudies-literatureUnknown224(12)Neuronal morphogenesis depends on extracellular guidance cues accurately instructing intracellular cytoskeletal remodeling. Here, we describe a novel role of the actin binding protein coronin 1A (Coro1A) in neuronal morphogenesis, where it mediates responses to the axon guidance cue netrin-1. We found that Coro1A localizes to growth cones and filopodial structures and is required for netrin-dependent axon turning, branching, and corpus callosum development. We previously discovered that Coro1A interacts with TRIM67, a brain-enriched E3 ubiquitin ligase that binds the netrin receptor DCC, and is also required for netrin-mediated neuronal morphogenesis. Loss of Coro1A and loss of TRIM67 shared similar phenotypes, suggesting that they may function together in the same netrin pathway. A Coro1AThe Journal of cell biologyCoro1A and TRIM67 collaborate in netrin-dependent neuronal morphogenesis.PMC12614643R35GM130312P30 CA016086R35GM135160R35 GM130312R35 GM135160906429Lukasik KEvans EBHo CTShah AGupton SLBear JETemple BO'Shaughnessy ECHsu CHfalseCoro1A and TRIM67 collaborate in netrin-dependent neuronal morphogenesis.Neuronal morphogenesis depends on extracellular guidance cues accurately instructing intracellular cytoskeletal remodeling. Here, we describe a novel role of the actin binding protein coronin 1A (Coro1A) in neuronal morphogenesis, where it mediates responses to the axon guidance cue netrin-1. We found that Coro1A localizes to growth cones and filopodial structures and is required for netrin-dependent axon turning, branching, and corpus callosum development. We previously discovered that Coro1A interacts with TRIM67, a brain-enriched E3 ubiquitin ligase that binds the netrin receptor DCC, and is also required for netrin-mediated neuronal morphogenesis. Loss of Coro1A and loss of TRIM67 shared similar phenotypes, suggesting that they may function together in the same netrin pathway. A Coro1A2025-01-01T00:00:00Z2025 Dec2026-06-05T12:34:40.908Z2026-05-16T03:12:40.661ZS-EPMC126146434108599510.1083/jcb.202503068