{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["McLeod D"],"funding":["Division of Emerging Frontiers","NHLBI NIH HHS","National Institutes of Health","Division of Chemical, Bioengineering, Environmental, and Transport Systems"],"pagination":["26"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12614770"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["25(3)"],"pubmed_abstract":["Biofilm infections represent a major public health threat due to their high tolerance to antimicrobials and the lack of specific anti-biofilm drugs. To develop such drugs, it is crucial to have high-throughput biofilm growth systems that can emulate in vivo conditions without the cost and complexity of animal models. However, no current biofilm reactor can provide in vivo-like conditions in a high throughput standard microtiter format. This paper demonstrates a novel high-throughput (HT) microfluidic perfusion biofilm reactor (HT-μPBR) compatible with a standard 96-well microtiter plate for in situ optical analysis. A snap-on liquid-tight cover for standard microtiter plates was designed and fabricated with fluidic channels to provide closed-loop recirculating perfusion. Our system takes s"],"journal":["Biomedical microdevices"],"pubmed_title":["A standard 96-well based high throughput microfluidic perfusion biofilm reactor for in situ optical analysis."],"pmcid":["PMC12614770"],"funding_grant_id":["1830941","R01 HL144157","R01HL144157","1743662"],"pubmed_authors":["Li Z","McLeod D","Wei L"],"additional_accession":[]},"is_claimable":false,"name":"A standard 96-well based high throughput microfluidic perfusion biofilm reactor for in situ optical analysis.","description":"Biofilm infections represent a major public health threat due to their high tolerance to antimicrobials and the lack of specific anti-biofilm drugs. To develop such drugs, it is crucial to have high-throughput biofilm growth systems that can emulate in vivo conditions without the cost and complexity of animal models. However, no current biofilm reactor can provide in vivo-like conditions in a high throughput standard microtiter format. This paper demonstrates a novel high-throughput (HT) microfluidic perfusion biofilm reactor (HT-μPBR) compatible with a standard 96-well microtiter plate for in situ optical analysis. A snap-on liquid-tight cover for standard microtiter plates was designed and fabricated with fluidic channels to provide closed-loop recirculating perfusion. Our system takes s","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023 Jul","modification":"2026-05-16T03:18:00.56Z","creation":"2026-05-16T03:12:57.478Z"},"accession":"S-EPMC12614770","cross_references":{"pubmed":["37493818"],"doi":["10.1007/s10544-023-00668-w"]}}