{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Henderson S"],"funding":["NIDDK NIH HHS","National Institute of Diabetes and Digestive and Kidney Diseases","Michigan State University"],"pagination":["1027-1037"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12619972"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["95(2)"],"pubmed_abstract":["This research reports on the preparation of a boron-doped diamond microelectrode modified with platinum nanoparticles and Nafion and its application for detecting nitric oxide (NO) <i>in vitro</i> in the mouse colon. Platinum nanoparticle deposition was performed potentiodynamically using a 2.0 mmol L<sup>-1</sup> potassium hexachloroplatinate solution and cycling from -0.2 to 1.3 V <i>vs</i> Ag/AgCl at 0.01 V s<sup>-1</sup> for 10 cycles. The Nafion overlayer was applied by immersion in a solution containing 2.5% (w/v) colloidal Nafion and drying overnight at 55 °C in a humid environment. The optimal microelectrode preparation conditions were chosen based on the electrode response for NO oxidation as well as rejection of nitrite (NO<sub>2</sub><sup>-</sup>) oxidation, the main interferent"],"journal":["Analytical chemistry"],"pubmed_title":["&lt;i&gt;In Vitro&lt;/i&gt; Monitoring of Nitric Oxide Release in the Mouse Colon Using a Boron-Doped Diamond Microelectrode Modified with Platinum Nanoparticles and Nafion."],"pmcid":["PMC12619972"],"funding_grant_id":["R01 DK12127201A1","AGR 2017-01280","R01 DK121272"],"pubmed_authors":["Galligan JJ","Swain GM","Perugachi V","Henderson S","Bhardwaj K","Espinoza-Montero P"],"additional_accession":[]},"is_claimable":false,"name":"&lt;i&gt;In Vitro&lt;/i&gt; Monitoring of Nitric Oxide Release in the Mouse Colon Using a Boron-Doped Diamond Microelectrode Modified with Platinum Nanoparticles and Nafion.","description":"This research reports on the preparation of a boron-doped diamond microelectrode modified with platinum nanoparticles and Nafion and its application for detecting nitric oxide (NO) <i>in vitro</i> in the mouse colon. Platinum nanoparticle deposition was performed potentiodynamically using a 2.0 mmol L<sup>-1</sup> potassium hexachloroplatinate solution and cycling from -0.2 to 1.3 V <i>vs</i> Ag/AgCl at 0.01 V s<sup>-1</sup> for 10 cycles. The Nafion overlayer was applied by immersion in a solution containing 2.5% (w/v) colloidal Nafion and drying overnight at 55 °C in a humid environment. The optimal microelectrode preparation conditions were chosen based on the electrode response for NO oxidation as well as rejection of nitrite (NO<sub>2</sub><sup>-</sup>) oxidation, the main interferent","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023 Jan","modification":"2026-06-05T14:34:26.969Z","creation":"2026-05-18T03:07:46.722Z"},"accession":"S-EPMC12619972","cross_references":{"pubmed":["36524968"],"doi":["10.1021/acs.analchem.2c03731"]}}