biostudies-literatureHenderson SNIDDK NIH HHSNational Institute of Diabetes and Digestive and Kidney DiseasesMichigan State University1027-1037https://www.ebi.ac.uk/biostudies/studies/S-EPMC12619972biostudies-literatureUnknown95(2)This research reports on the preparation of a boron-doped diamond microelectrode modified with platinum nanoparticles and Nafion and its application for detecting nitric oxide (NO) <i>in vitro</i> in the mouse colon. Platinum nanoparticle deposition was performed potentiodynamically using a 2.0 mmol L^-1 potassium hexachloroplatinate solution and cycling from -0.2 to 1.3 V <i>vs</i> Ag/AgCl at 0.01 V s^-1 for 10 cycles. The Nafion overlayer was applied by immersion in a solution containing 2.5% (w/v) colloidal Nafion and drying overnight at 55 °C in a humid environment. The optimal microelectrode preparation conditions were chosen based on the electrode response for NO oxidation as well as rejection of nitrite (NO₂^-) oxidation, the main interferentAnalytical chemistry&lt;i&gt;In Vitro&lt;/i&gt; Monitoring of Nitric Oxide Release in the Mouse Colon Using a Boron-Doped Diamond Microelectrode Modified with Platinum Nanoparticles and Nafion.PMC12619972R01 DK12127201A1AGR 2017-01280R01 DK121272Galligan JJSwain GMPerugachi VHenderson SBhardwaj KEspinoza-Montero Pfalse&lt;i&gt;In Vitro&lt;/i&gt; Monitoring of Nitric Oxide Release in the Mouse Colon Using a Boron-Doped Diamond Microelectrode Modified with Platinum Nanoparticles and Nafion.This research reports on the preparation of a boron-doped diamond microelectrode modified with platinum nanoparticles and Nafion and its application for detecting nitric oxide (NO) <i>in vitro</i> in the mouse colon. Platinum nanoparticle deposition was performed potentiodynamically using a 2.0 mmol L^-1 potassium hexachloroplatinate solution and cycling from -0.2 to 1.3 V <i>vs</i> Ag/AgCl at 0.01 V s^-1 for 10 cycles. The Nafion overlayer was applied by immersion in a solution containing 2.5% (w/v) colloidal Nafion and drying overnight at 55 °C in a humid environment. The optimal microelectrode preparation conditions were chosen based on the electrode response for NO oxidation as well as rejection of nitrite (NO₂^-) oxidation, the main interferent2023-01-01T00:00:00Z2023 Jan2026-06-05T14:34:26.969Z2026-05-18T03:07:46.722ZS-EPMC126199723652496810.1021/acs.analchem.2c03731