biostudies-literatureMao XShaanxi Provincial Science and Technology DepartmentNational Natural Science Foundation of ChinaXi&amp;apos;an Jiaotong University207-219https://www.ebi.ac.uk/biostudies/studies/S-EPMC12627352biostudies-literatureUnknown77<h4>Introduction</h4>Ferroptosis is an iron-dependent form of cell death triggered by the excessive accumulation of lipid peroxides. Understanding the regulatory mechanisms of ferroptosis and developing strategies to target this process hold significant clinical applications in tumor therapy.<h4>Objective</h4>Our study aims to search for novel candidate genes involved in the regulation of ferroptosis and to investigate their mechanism of action in ferroptosis and tumor therapy.<h4>Methods</h4>We employed a CRISPR-Cas9 library to perform a genome-wide screen under ferroptosis inducer treatment conditions, revealing Scavenger Receptor Class B Member 1(SCARB1) as a novel candidate gene involved in ferroptosis regulation. Subsequently, lipidomic analyses, metabolic interventions, and relevant Journal of advanced researchSCARB1 links cholesterol metabolism-mediated ferroptosis inhibition to radioresistance in tumor cells.PMC12627352821032722024JC-TBZC-1382372847QCYRCXM-2022-1662022KW-48QCYRCXM-2022-142Xiong JCai MWang BChen JWang CXiao ZZhang YMao XHe QHan SfalseSCARB1 links cholesterol metabolism-mediated ferroptosis inhibition to radioresistance in tumor cells.<h4>Introduction</h4>Ferroptosis is an iron-dependent form of cell death triggered by the excessive accumulation of lipid peroxides. Understanding the regulatory mechanisms of ferroptosis and developing strategies to target this process hold significant clinical applications in tumor therapy.<h4>Objective</h4>Our study aims to search for novel candidate genes involved in the regulation of ferroptosis and to investigate their mechanism of action in ferroptosis and tumor therapy.<h4>Methods</h4>We employed a CRISPR-Cas9 library to perform a genome-wide screen under ferroptosis inducer treatment conditions, revealing Scavenger Receptor Class B Member 1(SCARB1) as a novel candidate gene involved in ferroptosis regulation. Subsequently, lipidomic analyses, metabolic interventions, and relevant 2025-01-01T00:00:00Z2025 Nov2026-06-05T18:43:37.598Z2026-05-20T03:13:59.983ZS-EPMC126273523983272110.1016/j.jare.2025.01.026