{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Sceglovs A"],"funding":["Rigas Tehniska Universitate","H2020 Spreading Excellence and Widening Participation"],"pagination":["9916-9930"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12628331"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["8(11)"],"pubmed_abstract":["The growing threat of antimicrobial resistance has created an urgent demand for nonantibiotic biomaterials capable of preventing infections without promoting bacterial resistance. In this study, we developed injectable, covalently cross-linked hydrogels composed of ε-poly-l-lysine (ε-PL) and hyaluronic acid (HA) for localized wound infection treatment. These hydrogels combine the inherent antibacterial properties of ε-PL with the biocompatibility of HA, forming a shear-thinning, self-recovering system suitable for syringe-based administration. We first evaluated the antibacterial activity of pure ε-PL, determining minimum inhibitory and bactericidal concentrations (MIC/MBC) and evaluating resistance development against ATCC and clinically isolated multidrug-resistant strains (MRSA, ESBL-<i>E. coli</i>, <i>P. aeruginosa</i>). Notably, no resistance emerged in any strain after the serial passages. Hydrogels formed at varying ε-PL/HA ratios demonstrated strong immediate and long-term bactericidal activity while maintaining high cytocompatibility with murine and human fibroblasts. The hydrogels significantly reduced biofilm formation of <i>S. aureus</i> and MRSA within 24 h, achieving reductions comparable to or greater than vancomycin-gentamicin controls. Rheological analysis confirmed injectability, stability, and tunable stiffness. This study presents the first demonstration that ε-PL-based hydrogels can prevent resistance development in multidrug-resistant pathogens, offering a safe and antibiotic-free approach for infection control. The combination of antibacterial efficacy, resistance prevention, and biocompatibility makes these hydrogels promising candidates for wound infection management."],"journal":["ACS applied bio materials"],"pubmed_title":["Injectable ε-Polylysine/Hyaluronic Acid Hydrogels with Resistance-Preventing Antibacterial Activity for Treating Wound Infections."],"pmcid":["PMC12628331"],"funding_grant_id":["857287","C4835.Dok.1025 - 5.2.1.1.i.0/2/24/I/CFLA/003"],"pubmed_authors":["Sceglova M","Siverino C","Skadins I","Moriarty TF","Kroica J","Salma-Ancane K","Sceglovs A","Pirsko V"],"additional_accession":[]},"is_claimable":false,"name":"Injectable ε-Polylysine/Hyaluronic Acid Hydrogels with Resistance-Preventing Antibacterial Activity for Treating Wound Infections.","description":"The growing threat of antimicrobial resistance has created an urgent demand for nonantibiotic biomaterials capable of preventing infections without promoting bacterial resistance. In this study, we developed injectable, covalently cross-linked hydrogels composed of ε-poly-l-lysine (ε-PL) and hyaluronic acid (HA) for localized wound infection treatment. These hydrogels combine the inherent antibacterial properties of ε-PL with the biocompatibility of HA, forming a shear-thinning, self-recovering system suitable for syringe-based administration. We first evaluated the antibacterial activity of pure ε-PL, determining minimum inhibitory and bactericidal concentrations (MIC/MBC) and evaluating resistance development against ATCC and clinically isolated multidrug-resistant strains (MRSA, ESBL-<i>E. coli</i>, <i>P. aeruginosa</i>). Notably, no resistance emerged in any strain after the serial passages. Hydrogels formed at varying ε-PL/HA ratios demonstrated strong immediate and long-term bactericidal activity while maintaining high cytocompatibility with murine and human fibroblasts. The hydrogels significantly reduced biofilm formation of <i>S. aureus</i> and MRSA within 24 h, achieving reductions comparable to or greater than vancomycin-gentamicin controls. Rheological analysis confirmed injectability, stability, and tunable stiffness. This study presents the first demonstration that ε-PL-based hydrogels can prevent resistance development in multidrug-resistant pathogens, offering a safe and antibiotic-free approach for infection control. The combination of antibacterial efficacy, resistance prevention, and biocompatibility makes these hydrogels promising candidates for wound infection management.","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Nov","modification":"2026-05-19T03:23:04.981Z","creation":"2026-05-19T03:12:00.128Z"},"accession":"S-EPMC12628331","cross_references":{"pubmed":["41167198"],"doi":["10.1021/acsabm.5c01252"]}}