biostudies-literatureVliex LMMNWO-CCCDutch Research Council (NWO)2580693https://www.ebi.ac.uk/biostudies/studies/S-EPMC12629329biostudies-literatureUnknown17(1)Antibiotic-induced perturbations of the gut microbiome can be long-lasting and potentially affect host metabolic health. Strategies supporting microbial resilience are needed to mitigate the negative impact of antibiotics. We investigated the potential of 2'-fucosyllactose (2'-FL) supplementation after vancomycin use in a double-blind placebo-controlled randomized intervention among adults with overweight/obesity. Participants received oral vancomycin for seven days followed by 2'-FL or placebo for eight weeks. At baseline, after vancomycin use and after supplementation, glucose tolerance, insulin sensitivity, plasma lipids, glucagon-like peptide 1, inflammatory cytokines, fecal short-chain fatty acids (SCFAs) and branched-chain fatty acids were analyzed. Gut microbial diversity, compositiGut microbes2'-Fucosyllactose supplementation results in a transient improvement in gut microbial resilience after vancomycin use in adults with overweight or obesity: a randomized, double-blind, placebo-controlled intervention.PMC12629329ALWCC.2017.003Monzel ENauta ABlaak EEPenders JHolst JJBarnett DFassarella MZoetendal EGVliex LMMOudhuis GJfalse2'-Fucosyllactose supplementation results in a transient improvement in gut microbial resilience after vancomycin use in adults with overweight or obesity: a randomized, double-blind, placebo-controlled intervention.Antibiotic-induced perturbations of the gut microbiome can be long-lasting and potentially affect host metabolic health. Strategies supporting microbial resilience are needed to mitigate the negative impact of antibiotics. We investigated the potential of 2'-fucosyllactose (2'-FL) supplementation after vancomycin use in a double-blind placebo-controlled randomized intervention among adults with overweight/obesity. Participants received oral vancomycin for seven days followed by 2'-FL or placebo for eight weeks. At baseline, after vancomycin use and after supplementation, glucose tolerance, insulin sensitivity, plasma lipids, glucagon-like peptide 1, inflammatory cytokines, fecal short-chain fatty acids (SCFAs) and branched-chain fatty acids were analyzed. Gut microbial diversity, compositi2025-01-01T00:00:00Z2025 Dec2026-06-05T18:43:19.676Z2026-05-20T03:13:57.819ZS-EPMC126293294124344810.1080/19490976.2025.2580693