{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Zhang Y"],"funding":["National Natural Science Foundation of China"],"pagination":["e10624"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12631817"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["12(43)"],"pubmed_abstract":["Lanthanide-doped upconversion nanoparticles (UCNPs) are promising bioimaging probes due to their exceptional photostability and minimal background interference. However, their limited single-particle brightness has hindered broader applications. The study addresses this challenge by enhancing energy migration (EM) between sensitizer Yb<sup>3+</sup> to improve energy transfer efficiency to emitter Er<sup>3+</sup>. Nanoparticles are designed with a sensitizer/emitter-segregated core-shell-shell architecture (NaLu<sub>0.9</sub>Er<sub>0.1</sub>F<sub>4</sub>@NaYbF<sub>4</sub>@NaLuF<sub>4</sub>) to inhibit back energy transfer (BET) and then increased Yb<sup>3+</sup> doping levels (NaLu<sub>0.9-x</sub>Yb<sub>x</sub>Er<sub>0.1</sub>F<sub>4</sub>@NaYbF<sub>4</sub>@NaLuF<sub>4</sub>) to enhance EM into the core. UCNPs with an alloy-core of NaYb<sub>0.9</sub>Er<sub>0.1</sub>F<sub>4</sub> exhibited the brightest upconversion luminescence, achieving over a tenfold enhancement compared to NaLu<sub>0.9</sub>Er<sub>0.1</sub>F<sub>4</sub>-core counterparts, highlighting the importance of EM. Further optimization of the Yb<sup>3+</sup>/Er<sup>3+</sup> ratio and inert shell thickness (NaLuF<sub>4</sub>) maximized single-particle brightness. These optimized UCNPs enabled long-term tracking of axonal transport in live dorsal root ganglion (DRG) neurons. Using a Bayesian Hidden Markov Model, it quantitatively characterized resolved heterogeneous motion states and annotated trajectories with local spatiotemporal dynamics of retrograde, anterograde, and diffusive motions. The analysis revealed a kinesin-dynein coordination mechanism, where anterograde motion facilitates retrograde activation. It also examined the effects of inhibitors and stimulants on transport behavior. These findings establish upconversion single-particle tracking (uSPT) as a powerful tool for long-term, real-time monitoring of neuronal activities."],"journal":["Advanced science (Weinheim, Baden-Wurttemberg, Germany)"],"pubmed_title":["Enhanced Single-Particle Upconversion Imaging via Energy Migration Boosting."],"pmcid":["PMC12631817"],"funding_grant_id":["22174025","22474025"],"pubmed_authors":["Zhang W","Ding F","Zhai T","Ling H","Zhang Y","Liu Q","Wen R"],"additional_accession":[]},"is_claimable":false,"name":"Enhanced Single-Particle Upconversion Imaging via Energy Migration Boosting.","description":"Lanthanide-doped upconversion nanoparticles (UCNPs) are promising bioimaging probes due to their exceptional photostability and minimal background interference. However, their limited single-particle brightness has hindered broader applications. The study addresses this challenge by enhancing energy migration (EM) between sensitizer Yb<sup>3+</sup> to improve energy transfer efficiency to emitter Er<sup>3+</sup>. Nanoparticles are designed with a sensitizer/emitter-segregated core-shell-shell architecture (NaLu<sub>0.9</sub>Er<sub>0.1</sub>F<sub>4</sub>@NaYbF<sub>4</sub>@NaLuF<sub>4</sub>) to inhibit back energy transfer (BET) and then increased Yb<sup>3+</sup> doping levels (NaLu<sub>0.9-x</sub>Yb<sub>x</sub>Er<sub>0.1</sub>F<sub>4</sub>@NaYbF<sub>4</sub>@NaLuF<sub>4</sub>) to enhance EM into the core. UCNPs with an alloy-core of NaYb<sub>0.9</sub>Er<sub>0.1</sub>F<sub>4</sub> exhibited the brightest upconversion luminescence, achieving over a tenfold enhancement compared to NaLu<sub>0.9</sub>Er<sub>0.1</sub>F<sub>4</sub>-core counterparts, highlighting the importance of EM. Further optimization of the Yb<sup>3+</sup>/Er<sup>3+</sup> ratio and inert shell thickness (NaLuF<sub>4</sub>) maximized single-particle brightness. These optimized UCNPs enabled long-term tracking of axonal transport in live dorsal root ganglion (DRG) neurons. Using a Bayesian Hidden Markov Model, it quantitatively characterized resolved heterogeneous motion states and annotated trajectories with local spatiotemporal dynamics of retrograde, anterograde, and diffusive motions. The analysis revealed a kinesin-dynein coordination mechanism, where anterograde motion facilitates retrograde activation. It also examined the effects of inhibitors and stimulants on transport behavior. These findings establish upconversion single-particle tracking (uSPT) as a powerful tool for long-term, real-time monitoring of neuronal activities.","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Nov","modification":"2026-06-05T19:26:20.518Z","creation":"2026-05-20T03:14:51.811Z"},"accession":"S-EPMC12631817","cross_references":{"pubmed":["40859922"],"doi":["10.1002/advs.202510624"]}}