biostudies-literatureUnknown11(6)Suwittayarak RR.S. was supported by the Second Century Fund, Chulalongkorn University (C2F Ph.D. scholarship) and the 90th Anniversary of Chulalongkorn University Scholarship. T.O. was supported by the Faculty Research Fund, Faculty of Dentistry, Chulalongkorn University.<h4>Background</h4>To explore the cellular behavior of stem cells derived from human exfoliated deciduous teeth (SHED) in response to inorganic pyrophosphate (PP_i).<h4>Materials and methods</h4>SHED cells were isolated from the dental pulp tissues of human primary exfoliated teeth. Cell proliferation was examined using the MTT assay, colony-forming unit assay, and cell cycle analysis. Cell migration was evaluated using the scratch assay. Osteogenic differentiation was assessed by the expression of osteogenic marker genes and in vitro mineral deposition. Oil Red O staining was employed to determine intracellular lipid accumulation under adipogenic differentiation. For osteoclast differentiation, TRAP staining was used. The global gene expression profile was examined by RNA sequenClinical and experimental dental researche70248https://www.ebi.ac.uk/biostudies/studies/S-EPMC12631897biostudies-literaturePyrophosphate Regulates Multilineage Differentiation in Stem Cells From Human Exfoliated Deciduous Teeth.PMC12631897Kornsuthisopon CNowwarote NOsathanon TFoster BLSuwittayarak RSukarawan WEgusa HfalsePyrophosphate Regulates Multilineage Differentiation in Stem Cells From Human Exfoliated Deciduous Teeth.<h4>Background</h4>To explore the cellular behavior of stem cells derived from human exfoliated deciduous teeth (SHED) in response to inorganic pyrophosphate (PP_i).<h4>Materials and methods</h4>SHED cells were isolated from the dental pulp tissues of human primary exfoliated teeth. Cell proliferation was examined using the MTT assay, colony-forming unit assay, and cell cycle analysis. Cell migration was evaluated using the scratch assay. Osteogenic differentiation was assessed by the expression of osteogenic marker genes and in vitro mineral deposition. Oil Red O staining was employed to determine intracellular lipid accumulation under adipogenic differentiation. For osteoclast differentiation, TRAP staining was used. The global gene expression profile was examined by RNA sequen2025-01-01T00:00:00Z2025 Dec2026-05-19T03:18:29.39Z2026-05-19T03:11:55.092ZS-EPMC126318974126349410.1002/cre2.70248