{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Tutanov OS"],"funding":["National Institutes of Health National Cancer Institute","NEI NIH HHS","NIDDK NIH HHS","NHLBI NIH HHS","NCI NIH HHS","NIH HHS","National Science Foundation"],"pagination":["116287"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12632813"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["44(10)"],"pubmed_abstract":["Biofluids contain a heterogeneous mixture of extracellular vesicles and non-vesicular nanoparticles (including exomeres and supermeres) that transport a diverse array of proteins, RNA, and lipids. Our previous efforts to characterize the contents of these carriers in colorectal cancer relied on 2D culture systems requiring large-scale setups and time-consuming ultracentrifugation-based isolation. To streamline this process, we have combined 3D hollow-fiber bioreactor production and fast-protein liquid chromatography-based size-exclusion chromatography. Here, we compare the impact of culture methods and purification strategies on small extracellular vesicle, exomere, and supermere cargo. Proteomic analyses show consistently distinct profiles for extracellular vesicles, exomeres, and superme"],"journal":["Cell reports"],"pubmed_title":["A comprehensive analysis of supermere, exomere, and extracellular vesicle isolation and cargo in colorectal cancer."],"pmcid":["PMC12632813"],"funding_grant_id":["P30 DK020593","P01 CA229123","UH3 CA241685","P01 HL116263","P30 CA068485","R01 HL173598","U24 DK059637","P50 CA236733","P30 DK058404","P30 EY008126","R35 CA197570","S10 OD034315"],"pubmed_authors":["Castleberry M","John E","Coffey RJ","Michell DL","Patton JG","Massick C","Jeppesen DK","Weaver AM","Liu Q","Hamilton MS","Vickers KC","Ramirez M","Zhang Q","Franklin JL","Patel P","Tutanov OS","Higginbotham JN","Glass SE","Cao Z","Chen HC","Rose KL","Krystofiak E","Jimenez L","Sheng Q"],"additional_accession":[]},"is_claimable":false,"name":"A comprehensive analysis of supermere, exomere, and extracellular vesicle isolation and cargo in colorectal cancer.","description":"Biofluids contain a heterogeneous mixture of extracellular vesicles and non-vesicular nanoparticles (including exomeres and supermeres) that transport a diverse array of proteins, RNA, and lipids. Our previous efforts to characterize the contents of these carriers in colorectal cancer relied on 2D culture systems requiring large-scale setups and time-consuming ultracentrifugation-based isolation. To streamline this process, we have combined 3D hollow-fiber bioreactor production and fast-protein liquid chromatography-based size-exclusion chromatography. Here, we compare the impact of culture methods and purification strategies on small extracellular vesicle, exomere, and supermere cargo. Proteomic analyses show consistently distinct profiles for extracellular vesicles, exomeres, and superme","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Oct","modification":"2026-06-10T05:17:29.902Z","creation":"2026-06-10T03:07:21.708Z"},"accession":"S-EPMC12632813","cross_references":{"pubmed":["40966084"],"doi":["10.1016/j.celrep.2025.116287"]}}