biostudies-literatureUnknown15(10)Balkrishna A<i>Rauvolfia serpentina</i> (L.) Benth. Ex Kurz is a greatly appreciated medicinal plant, well-known for its therapeutic benefits in traditional medicine, particularly in Ayurveda, where the roots and whole plant are used to treat a variety of ailments. However, studies focusing on <i>R. serpentina</i> seeds are relatively scarce. Hence, the present study provides a novel approach by analysing the seed oil of <i>R. serpentina</i> extracted using the supercritical-carbon dioxide-fluid-extraction (SCFE) technique. The research employed advanced analytical methods including gas-chromatography with flame ionization detector (GC-FID), gas-chromatography-tandem mass spectrometry (GC-MS/MS), and high performance thin layer chromatography (HPTLC) to characterise the chemical composition of the extJournal of pharmaceutical analysis101299https://www.ebi.ac.uk/biostudies/studies/S-EPMC12634849biostudies-literatureIntegrating gas-chromatographical analyses with nuclear-magnetic-resonance spectroscopy to elucidate anti-microbial profile of oleoresins isolated from &lt;i&gt;Rauvolfia serpentina&lt;/i&gt; seeds by supercritical-(CO&lt;sub&gt;2&lt;/sub&gt;)-fluid extraction.PMC12634849Joshi MVarshney AJangid HBalkrishna AKabdwal MNain PPriya Rani MVarshney YLochab SfalseIntegrating gas-chromatographical analyses with nuclear-magnetic-resonance spectroscopy to elucidate anti-microbial profile of oleoresins isolated from &lt;i&gt;Rauvolfia serpentina&lt;/i&gt; seeds by supercritical-(CO&lt;sub&gt;2&lt;/sub&gt;)-fluid extraction.<i>Rauvolfia serpentina</i> (L.) Benth. Ex Kurz is a greatly appreciated medicinal plant, well-known for its therapeutic benefits in traditional medicine, particularly in Ayurveda, where the roots and whole plant are used to treat a variety of ailments. However, studies focusing on <i>R. serpentina</i> seeds are relatively scarce. Hence, the present study provides a novel approach by analysing the seed oil of <i>R. serpentina</i> extracted using the supercritical-carbon dioxide-fluid-extraction (SCFE) technique. The research employed advanced analytical methods including gas-chromatography with flame ionization detector (GC-FID), gas-chromatography-tandem mass spectrometry (GC-MS/MS), and high performance thin layer chromatography (HPTLC) to characterise the chemical composition of the ext2025-01-01T00:00:00Z2025 Oct2026-06-05T18:35:58.152Z2026-05-20T03:12:30.045ZS-EPMC126348494128233210.1016/j.jpha.2025.101299