{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Li Z"],"funding":["Natural Science Foundation of Hebei Provenience","Health Commission of Hebei Province","Department of Education of Hebei Pro","Hebei Provincial Key Research Projects","Hebei Province Outstanding youth fund"],"pagination":["40958"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12635151"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["15(1)"],"pubmed_abstract":["Breast cancer (BC) is the most common malignancy among women, with its progression and prognosis significantly influenced by the tumor microenvironment (TME). Age-related differences in TME composition lead to distinct tumor behaviors: young patients (≤ 40 years) exhibit aggressive tumors, while elderly patients (> 70 years) experience immunosenescence and reduced therapy responses. We performed single-cell RNA sequencing (scRNA-seq) analysis on tumors from 10 breast cancer patients (5 ≤ 40 years, 5 ≥ 70 years), encompassing 33,664 high-quality cells. After cell annotation and batch correction, malignant epithelial cells were identified using inferCNV. We applied pseudotime trajectory analysis, pathway enrichment, and cell-cell communication profiling to investigate age-specific TME dynami"],"journal":["Scientific reports"],"pubmed_title":["Single-cell RNA sequencing reveals age-related heterogeneity in the tumor microenvironment of breast cancer patients."],"pmcid":["PMC12635151"],"funding_grant_id":["H2022206418","No.BJ2025056","No.22372409D","ZF2025023","20240643","CXZZBS2020111"],"pubmed_authors":["Kou S","Li Z","Feng J","An M","Liu B","Yu J","Shang F","Zhao Z"],"additional_accession":[]},"is_claimable":false,"name":"Single-cell RNA sequencing reveals age-related heterogeneity in the tumor microenvironment of breast cancer patients.","description":"Breast cancer (BC) is the most common malignancy among women, with its progression and prognosis significantly influenced by the tumor microenvironment (TME). Age-related differences in TME composition lead to distinct tumor behaviors: young patients (≤ 40 years) exhibit aggressive tumors, while elderly patients (> 70 years) experience immunosenescence and reduced therapy responses. We performed single-cell RNA sequencing (scRNA-seq) analysis on tumors from 10 breast cancer patients (5 ≤ 40 years, 5 ≥ 70 years), encompassing 33,664 high-quality cells. After cell annotation and batch correction, malignant epithelial cells were identified using inferCNV. We applied pseudotime trajectory analysis, pathway enrichment, and cell-cell communication profiling to investigate age-specific TME dynami","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Nov","modification":"2026-05-19T03:23:54.808Z","creation":"2026-05-19T03:12:07.14Z"},"accession":"S-EPMC12635151","cross_references":{"pubmed":["41266463"],"doi":["10.1038/s41598-025-24720-2"]}}