{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"submitter":["Cordoza JL"],"funding":["NIGMS NIH HHS"],"pubmed_abstract":["Actinobacteria are a rich source of bioactive compounds and unique biosynthetic chemistry. <i>Micromonospora echinospora</i> subsp. <i>challisensis</i> NRRL 12255 produces the aromatic polyketide TLN-05220, which exhibits nanomolar activity against antibiotic-resistant human pathogens including vancomycin-resistant <i>Enterococcus faecalis</i> and methicillin-resistant <i>Staphylococcus aureus</i>. The pentangular polyphenol core of TLN-05220 is decorated with a piperazinone moiety, yet the enzymes responsible for the construction of this uncommon modification from amino acid precursors are unknown. Synthetic piperazinone-containing molecules have diverse antimicrobial, antiviral, anticancer, and anti-inflammatory bioactivity profiles, and determining biosynthetic routes for the assembly o"],"journal":["bioRxiv : the preprint server for biology"],"pagination":["2025.10.30.685158"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12636320"],"repository":["biostudies-literature"],"pubmed_title":["Integrated metabolomic and genomic insights into amino acid incorporation within the hybrid polyketide-alkaloid antibiotic TLN-05220."],"pmcid":["PMC12636320"],"funding_grant_id":["R01 GM125943"],"pubmed_authors":["Sanchez LM","Nicholas JC","Horta CA","Owen DM","Calhoun JT","Rodriguez-Velandia L","Cordoza JL","McKinnie SMK","Johnson RA","Watts KR","Luu GT","Petukhova VZ","Whiteley AE"],"additional_accession":[]},"is_claimable":false,"name":"Integrated metabolomic and genomic insights into amino acid incorporation within the hybrid polyketide-alkaloid antibiotic TLN-05220.","description":"Actinobacteria are a rich source of bioactive compounds and unique biosynthetic chemistry. <i>Micromonospora echinospora</i> subsp. <i>challisensis</i> NRRL 12255 produces the aromatic polyketide TLN-05220, which exhibits nanomolar activity against antibiotic-resistant human pathogens including vancomycin-resistant <i>Enterococcus faecalis</i> and methicillin-resistant <i>Staphylococcus aureus</i>. The pentangular polyphenol core of TLN-05220 is decorated with a piperazinone moiety, yet the enzymes responsible for the construction of this uncommon modification from amino acid precursors are unknown. Synthetic piperazinone-containing molecules have diverse antimicrobial, antiviral, anticancer, and anti-inflammatory bioactivity profiles, and determining biosynthetic routes for the assembly o","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Oct","modification":"2026-06-19T03:17:02.012Z","creation":"2026-06-19T03:07:09.118Z"},"accession":"S-EPMC12636320","cross_references":{"pubmed":["41279854"],"doi":["10.1101/2025.10.30.685158"]}}