biostudies-literatureUnknownMoro NNICHD NIH HHSNHLBI NIH HHSNIGMS NIH HHSCraniofacial Developmental Protein 1 (CFDP1) is a member of the evolutionarily conserved family of Bucentaur (BCNT) proteins and was originally classified as a protein required for cell survival and differentiation during tooth development. Yeast Swc5, a BCNT family member, is an essential subunit of the yeast SWR1C chromatin remodeling complex that catalyzes the deposition of histone variant H2A.Z. Direct connections between CFDP1, H2A.Z deposition, and the mammalian SWR1 homolog, Snf2-Related CREBBP Activator Protein (SRCAP), have not been identified. Here, we perform detailed biochemical reconstitution and characterization of the human SRCAP complex (SRCAP-C). We find that CFDP1 weakly interacts with SRCAP-C in a salt concentration-dependent manner. SRCAP-C purified under a high-salt cobioRxiv : the preprint server for biology2025.10.30.685587https://www.ebi.ac.uk/biostudies/studies/S-EPMC12636367biostudies-literatureCFDP1 is required for histone variant H2A.Z deposition by the human SRCAP chromatin remodeling complex.PMC12636367R03 HD095088R01 GM134130R01 HL140471R35 GM122519Butty VLWatanabe SBoyer LAMoro NYang DLevine SSPeterson CLfalseCFDP1 is required for histone variant H2A.Z deposition by the human SRCAP chromatin remodeling complex.Craniofacial Developmental Protein 1 (CFDP1) is a member of the evolutionarily conserved family of Bucentaur (BCNT) proteins and was originally classified as a protein required for cell survival and differentiation during tooth development. Yeast Swc5, a BCNT family member, is an essential subunit of the yeast SWR1C chromatin remodeling complex that catalyzes the deposition of histone variant H2A.Z. Direct connections between CFDP1, H2A.Z deposition, and the mammalian SWR1 homolog, Snf2-Related CREBBP Activator Protein (SRCAP), have not been identified. Here, we perform detailed biochemical reconstitution and characterization of the human SRCAP complex (SRCAP-C). We find that CFDP1 weakly interacts with SRCAP-C in a salt concentration-dependent manner. SRCAP-C purified under a high-salt co2025-01-01T00:00:00Z2025 Oct2026-06-11T03:18:56.157Z2026-06-11T03:08:23.713ZS-EPMC126363674127897810.1101/2025.10.30.685587