biostudies-literatureTakemura AYamaha Motor Co LtdJapan Society for the Promotion of Science102341https://www.ebi.ac.uk/biostudies/studies/S-EPMC12639397biostudies-literatureUnknown44Mild hyperbaric oxygen (MHO) attenuates the muscle atrophy caused by muscle disuse. Training cessation results in the partial or complete loss of training-induced adaptations, including mitochondrial enzyme activities. The present study aimed to determine whether exposure to MHO after running training attenuated negative adaptation induced by detraining. We allocated eight-week-old mice into training (Tr), detraining after the training period (DeTr), and detraining + mild hyperbaric oxygen (DeTr + MHO, 1.3 atm absolute with 38 % oxygen) groups. Mice underwent voluntary wheel running for four weeks, followed by a two-week detraining period under normal or MHO conditions. The soleus muscle weight (mg/g BW) decreased by approximately 30 % in the DeTr and DeTr + MHO groups compared to the Tr gBiochemistry and biophysics reportsMild hyperbaric oxygen does not attenuate mitochondrial decrease induced by detraining in mice.PMC1263939724K20722Egawa TUemichi KFujita SHayashi TIyama ROba MTakemura AZhao HfalseMild hyperbaric oxygen does not attenuate mitochondrial decrease induced by detraining in mice.Mild hyperbaric oxygen (MHO) attenuates the muscle atrophy caused by muscle disuse. Training cessation results in the partial or complete loss of training-induced adaptations, including mitochondrial enzyme activities. The present study aimed to determine whether exposure to MHO after running training attenuated negative adaptation induced by detraining. We allocated eight-week-old mice into training (Tr), detraining after the training period (DeTr), and detraining + mild hyperbaric oxygen (DeTr + MHO, 1.3 atm absolute with 38 % oxygen) groups. Mice underwent voluntary wheel running for four weeks, followed by a two-week detraining period under normal or MHO conditions. The soleus muscle weight (mg/g BW) decreased by approximately 30 % in the DeTr and DeTr + MHO groups compared to the Tr g2025-01-01T00:00:00Z2025 Dec2026-06-01T03:20:18.939Z2026-06-01T03:11:03.622ZS-EPMC126393974128254210.1016/j.bbrep.2025.102341