biostudies-literatureUnknown60(10)Krajewski A<h4>Aim</h4>The objective of this systematic review and meta-analysis was to evaluate the influence of non-surgical periodontal therapy (NSPT) on subgingival microbiota in systemically healthy patients with periodontitis.<h4>Methods</h4>A systematic literature search was conducted in Medline, Embase, LILACS, and the Cochrane Library, supplemented by a manual search for studies investigating the effect of NSPT on subgingival microbiota in systemically healthy patients.<h4>Results</h4>In this review, 115 studies were included, of which 41 used checkerboard DNA-DNA hybridisation technology, 30 real-time quantitative PCR, 36 bacterial culture, and 8 16S ribosomal ribonucleic acid (rRNA) gene sequencing technology. Meta-analyses showed a significant decrease in mean counts of Aggregatibacter acJournal of periodontal research963-993https://www.ebi.ac.uk/biostudies/studies/S-EPMC12640219biostudies-literatureThe Effect of Non-Surgical Periodontal Therapy on Subgingival Microbiota: A Systematic Review and Meta-Analysis.PMC12640219Ercal PDonos NPerussolo JGkranias NKrajewski AfalseThe Effect of Non-Surgical Periodontal Therapy on Subgingival Microbiota: A Systematic Review and Meta-Analysis.<h4>Aim</h4>The objective of this systematic review and meta-analysis was to evaluate the influence of non-surgical periodontal therapy (NSPT) on subgingival microbiota in systemically healthy patients with periodontitis.<h4>Methods</h4>A systematic literature search was conducted in Medline, Embase, LILACS, and the Cochrane Library, supplemented by a manual search for studies investigating the effect of NSPT on subgingival microbiota in systemically healthy patients.<h4>Results</h4>In this review, 115 studies were included, of which 41 used checkerboard DNA-DNA hybridisation technology, 30 real-time quantitative PCR, 36 bacterial culture, and 8 16S ribosomal ribonucleic acid (rRNA) gene sequencing technology. Meta-analyses showed a significant decrease in mean counts of Aggregatibacter ac2025-01-01T00:00:00Z2025 Oct2026-06-05T17:22:49.211Z2026-05-19T03:12:04.882ZS-EPMC126402194034703910.1111/jre.13409