biostudies-literatureZheng ZGuangzhou National Laboratorye0336479https://www.ebi.ac.uk/biostudies/studies/S-EPMC12643299biostudies-literatureUnknown20(11)Hydrated electrons (eaq-) are widely studied in pollutant degradation owing to their high reducing power. Recent studies indicate that transiently generated eaq- during radiotherapy can enhance chemotherapeutic antitumor effects via reduction activity. However, biomedical applications remain limited because conventional methods generate eaq- in situ and are short-lived, precluding storage. In this study, we present a storable electromagnetic base liquid (EBL), reference-linked to prior preparation work, and analyze its physicochemical and organism-level effects. The EBL showed strong alkalinity (pH 13.08), low oxidation-reduction potential (ORP 47.1 mV), and a total antioxidant capacity of 1.6 mM Trolox equivalents antioxidant capacity. Using a larva zebrafish (Danio rerio) digital phenotyping platform, we identified a non-adverse concentration and generated unbiased predictions clustering EBL with antitumor, uric acid-lowering, and hypoglycemic drugs. These findings motivate further biological investigation of EBL's biomedical potential and provide a basis for subsequent validation.PloS oneNew potentials in biomedical application of hydrated electrons: A functional and biomedical effects study of an electromagnetic base liquid containing hydrated electrons.PMC12643299GZNL2025C01009Zheng ZLi XRan FZhang YLiang LZhang LBi LfalseNew potentials in biomedical application of hydrated electrons: A functional and biomedical effects study of an electromagnetic base liquid containing hydrated electrons.Hydrated electrons (eaq-) are widely studied in pollutant degradation owing to their high reducing power. Recent studies indicate that transiently generated eaq- during radiotherapy can enhance chemotherapeutic antitumor effects via reduction activity. However, biomedical applications remain limited because conventional methods generate eaq- in situ and are short-lived, precluding storage. In this study, we present a storable electromagnetic base liquid (EBL), reference-linked to prior preparation work, and analyze its physicochemical and organism-level effects. The EBL showed strong alkalinity (pH 13.08), low oxidation-reduction potential (ORP 47.1 mV), and a total antioxidant capacity of 1.6 mM Trolox equivalents antioxidant capacity. Using a larva zebrafish (Danio rerio) digital phenotyping platform, we identified a non-adverse concentration and generated unbiased predictions clustering EBL with antitumor, uric acid-lowering, and hypoglycemic drugs. These findings motivate further biological investigation of EBL's biomedical potential and provide a basis for subsequent validation.2025-01-01T00:00:00Z20252026-06-06T03:16:12.009Z2026-06-06T03:06:57.565ZS-EPMC126432994128467510.1371/journal.pone.0336479