biostudies-literatureUnknown16Budkina A<h4>Background</h4>CHASERR, a conserved long non-coding RNA located upstream of CHD2, transcriptionally represses CHD2 in <i>cis</i>. Both genes are highly expressed in lymphocytes, suggesting roles in immune regulation, though their functions remain undefined.<h4>Results</h4>We identified elevated expression of CHASERR and CHD2 in naïve and regulatory T cells through analysis of single-cell and bulk RNA-seq datasets. Both their promoters are bound by FOXP3, the key regulator of Treg cells, and FOXP1, the key regulator of naïve T cell quiescence. Expression dynamics during early T cell activation revealed that a decline in CHASERR precedes a transient increase in CHD2. Correlation analysis linked CHASERR/CHD2 expression to quiescence-associated genes, suggesting a role in maintaining T celFrontiers in immunology1652359https://www.ebi.ac.uk/biostudies/studies/S-EPMC12644095biostudies-literatureCHASERR-CHD2 dynamics in T cell quiescence and its modulation by cyclosporine.PMC12644095Loguinova MYSergeev NAMarakulina DBudkina AZubritskiy AMedvedeva YAfalseCHASERR-CHD2 dynamics in T cell quiescence and its modulation by cyclosporine.<h4>Background</h4>CHASERR, a conserved long non-coding RNA located upstream of CHD2, transcriptionally represses CHD2 in <i>cis</i>. Both genes are highly expressed in lymphocytes, suggesting roles in immune regulation, though their functions remain undefined.<h4>Results</h4>We identified elevated expression of CHASERR and CHD2 in naïve and regulatory T cells through analysis of single-cell and bulk RNA-seq datasets. Both their promoters are bound by FOXP3, the key regulator of Treg cells, and FOXP1, the key regulator of naïve T cell quiescence. Expression dynamics during early T cell activation revealed that a decline in CHASERR precedes a transient increase in CHD2. Correlation analysis linked CHASERR/CHD2 expression to quiescence-associated genes, suggesting a role in maintaining T cel2025-01-01T00:00:00Z20252026-06-05T18:00:13.931Z2026-05-20T03:09:33.375ZS-EPMC126440954130696910.3389/fimmu.2025.1652359