biostudies-literatureKang MMinistry of Science and Technology of the People's Republic of China (Chinese Ministry of Science and Technology)857https://www.ebi.ac.uk/biostudies/studies/S-EPMC12644464biostudies-literatureUnknown16(1)Podocytopathy is an emerging global health concern characterized by the injury of podocytes through various direct or indirect mechanisms. Recent research has highlighted a potential link between podocyte loss and various programmed cell death pathways, while the precise mechanisms of podocyte injury remain ambiguous. We conducted single-nucleus RNA sequencing (snRNA-seq) on kidney tissues from adriamycin-induced nephropathy (AN) mice (BALB/c, male) and renal biopsy samples from patients with different types of podocytopathy, such as focal segmental glomerulosclerosis (FSGS), minimal change disease (MCD) and obesity-related glomerulopathy (ORG). We found podocytes in diseased groups exhibited elevated ferroptosis scores based on the gene module score of programmed cell death pathways. TargCell death & diseaseElovl7 sensitizes podocytes to ferroptosis in podocytopathy by elongating polyunsaturated fatty acids.PMC126444642022YFC2505400Zhou LWu JYan PZhan XQing JLi QZhao YLi MMao JLu MWen JZhu JWei RHuang XKang MBai LWang XLiu FfalseElovl7 sensitizes podocytes to ferroptosis in podocytopathy by elongating polyunsaturated fatty acids.Podocytopathy is an emerging global health concern characterized by the injury of podocytes through various direct or indirect mechanisms. Recent research has highlighted a potential link between podocyte loss and various programmed cell death pathways, while the precise mechanisms of podocyte injury remain ambiguous. We conducted single-nucleus RNA sequencing (snRNA-seq) on kidney tissues from adriamycin-induced nephropathy (AN) mice (BALB/c, male) and renal biopsy samples from patients with different types of podocytopathy, such as focal segmental glomerulosclerosis (FSGS), minimal change disease (MCD) and obesity-related glomerulopathy (ORG). We found podocytes in diseased groups exhibited elevated ferroptosis scores based on the gene module score of programmed cell death pathways. Targ2025-01-01T00:00:00Z2025 Nov2026-06-05T19:07:57.576Z2026-05-20T03:14:53.365ZS-EPMC126444644128571610.1038/s41419-025-08144-4