biostudies-literatureUnknown133(11)Lee HJ<h4>Background</h4>Cancer metastasis, primarily driven by epithelial-to-mesenchymal transition (EMT), is responsible for most cancer-related mortalities. Traditional pre-clinical models fail to fully capture mesenchymal characteristics due to the loss of human stroma. The extracellular matrix (ECM) plays a crucial role in EMT, yet conventional in vitro models often rely on defined ECM components, which may not adequately replicate the human physiological ECM niche.<h4>Methods</h4>To mimic the in situ dissemination of cancer cells, we employed a patient-derived extracellular matrix (pdECM). We transitioned the culture matrix for patient-derived colorectal cancer organoids from a basement membrane extract (BME) to a patient-derived ECM (pdECM). We performed single-cell multiomic analyses, inBritish journal of cancer1708-1719https://www.ebi.ac.uk/biostudies/studies/S-EPMC12644670biostudies-literatureSingle-cell multiomic modelling of early metastatic events promoted by the extracellular matrix.PMC12644670Park JECho YKim TYPark JChoi JKKim KPark WYKwon JSong SHKim PKim YJLee HJKang JLee JHfalseSingle-cell multiomic modelling of early metastatic events promoted by the extracellular matrix.<h4>Background</h4>Cancer metastasis, primarily driven by epithelial-to-mesenchymal transition (EMT), is responsible for most cancer-related mortalities. Traditional pre-clinical models fail to fully capture mesenchymal characteristics due to the loss of human stroma. The extracellular matrix (ECM) plays a crucial role in EMT, yet conventional in vitro models often rely on defined ECM components, which may not adequately replicate the human physiological ECM niche.<h4>Methods</h4>To mimic the in situ dissemination of cancer cells, we employed a patient-derived extracellular matrix (pdECM). We transitioned the culture matrix for patient-derived colorectal cancer organoids from a basement membrane extract (BME) to a patient-derived ECM (pdECM). We performed single-cell multiomic analyses, in2025-01-01T00:00:00Z2025 Dec2026-06-06T15:46:38.247Z2026-06-02T03:09:33.37ZS-EPMC126446704095425710.1038/s41416-025-03181-4