{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["16(1)"],"submitter":["Rahimov F"],"pubmed_abstract":["Interleukin-1β (IL-1β) is dysregulated in chronic inflammatory diseases, yet the genetic factors influencing IL-1β production remain largely unknown. Myeloid-derived cells are the primary producers of IL-1β, which prompted a genome-wide CRISPR knockout screen in the human myeloid-derived U937 cells treated with lipopolysaccharide (LPS) to mimic inflammatory conditions and sorted for high and low intracellular IL-1β levels. A total of 295 genes are identified as regulators of IL-1β production, with 57 overlapping loci associated with inflammatory diseases, including the TNRC18 gene locus associated with multiple diseases in the Finnish population. U937 cells engineered with the Finnish-enriched rs748670681 risk allele demonstrate decreased expression of TNRC18 and an adjacent gene WIPI2, re"],"journal":["Nature communications"],"pagination":["10346"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12644769"],"repository":["biostudies-literature"],"pubmed_title":["A genome-wide CRISPR screen identifies the TNRC18 gene locus as a regulator of inflammatory signaling."],"pmcid":["PMC12644769"],"pubmed_authors":["Shi M","den Hollander AI","Espinoza CA","Waring JF","Rahimov F","Wang J","Mahi NA","Schmidt M","Modi A","Avram V","Anton T","Verduzco D","Singh S","Katlinski KV","Nyamugenda E","Macoritto M","Tam J","Lu C","Chung N","Biswas A","Stender JD","Ghosh S","Keller A","Flister MJ","Petiwala S"],"additional_accession":[]},"is_claimable":false,"name":"A genome-wide CRISPR screen identifies the TNRC18 gene locus as a regulator of inflammatory signaling.","description":"Interleukin-1β (IL-1β) is dysregulated in chronic inflammatory diseases, yet the genetic factors influencing IL-1β production remain largely unknown. Myeloid-derived cells are the primary producers of IL-1β, which prompted a genome-wide CRISPR knockout screen in the human myeloid-derived U937 cells treated with lipopolysaccharide (LPS) to mimic inflammatory conditions and sorted for high and low intracellular IL-1β levels. A total of 295 genes are identified as regulators of IL-1β production, with 57 overlapping loci associated with inflammatory diseases, including the TNRC18 gene locus associated with multiple diseases in the Finnish population. U937 cells engineered with the Finnish-enriched rs748670681 risk allele demonstrate decreased expression of TNRC18 and an adjacent gene WIPI2, re","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Nov","modification":"2026-06-05T18:52:20.829Z","creation":"2026-05-20T03:14:14.058Z"},"accession":"S-EPMC12644769","cross_references":{"pubmed":["41285767"],"doi":["10.1038/s41467-025-65277-y"]}}