{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Oka T"],"funding":["Ministry of Health, Labour and Welfare","Japan Agency for Medical Research and Development","SHIONOGI INFECTIOUS DISEASE RESEARCH PROMOTION FOUNDATION","Japan Society for the Promotion of Science"],"pagination":["e0132525"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12646008"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["99(11)"],"pubmed_abstract":["Human sapoviruses (HuSaVs), which cause acute gastroenteritis, are highly diverse. Fifteen HuSaV genotype strains (GI.1-7, GII.1-5, -8, and GV.1-2) were efficiently propagated in the human duodenum-derived cell line HuTu80 when supplemented with conjugated bile acid (T. Oka, T.-C. Li, K. Yonemitsu, Y. Ami, et al., J Virol 98:e00639-24, 2024, https://doi.org/10.1128/jvi.00639-24). However, the host cellular factors involved in HuSaV infection and propagation remain unidentified. Using a knockout approach, we newly identified and confirmed that CD36 is a critical cellular protein for the propagation of all 15 HuSaV genotypes tested in HuTu80 cells. When the CD36 gene was re-expressed in CD36 gene knockout HuTu80 cells, all HuSaV genotype strains, except for GI.6 and GII.3, recovered their vi"],"journal":["Journal of virology"],"pubmed_title":["CD36 is required for human sapovirus propagation."],"pmcid":["PMC12646008"],"funding_grant_id":["JP23fk0108683","JP23K21362","JP24K13428","JPMH22KA1001","JPMH25KA1001","JP20K08320"],"pubmed_authors":["Takagi H","Oka T","Okemoto-Nakamura Y"],"additional_accession":[]},"is_claimable":false,"name":"CD36 is required for human sapovirus propagation.","description":"Human sapoviruses (HuSaVs), which cause acute gastroenteritis, are highly diverse. Fifteen HuSaV genotype strains (GI.1-7, GII.1-5, -8, and GV.1-2) were efficiently propagated in the human duodenum-derived cell line HuTu80 when supplemented with conjugated bile acid (T. Oka, T.-C. Li, K. Yonemitsu, Y. Ami, et al., J Virol 98:e00639-24, 2024, https://doi.org/10.1128/jvi.00639-24). However, the host cellular factors involved in HuSaV infection and propagation remain unidentified. Using a knockout approach, we newly identified and confirmed that CD36 is a critical cellular protein for the propagation of all 15 HuSaV genotypes tested in HuTu80 cells. When the CD36 gene was re-expressed in CD36 gene knockout HuTu80 cells, all HuSaV genotype strains, except for GI.6 and GII.3, recovered their vi","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Nov","modification":"2026-06-05T19:07:39.388Z","creation":"2026-05-20T03:14:51.077Z"},"accession":"S-EPMC12646008","cross_references":{"pubmed":["41190813"],"doi":["10.1128/jvi.01325-25"]}}