biostudies-literatureOka TMinistry of Health, Labour and WelfareJapan Agency for Medical Research and DevelopmentSHIONOGI INFECTIOUS DISEASE RESEARCH PROMOTION FOUNDATIONJapan Society for the Promotion of Sciencee0132525https://www.ebi.ac.uk/biostudies/studies/S-EPMC12646008biostudies-literatureUnknown99(11)Human sapoviruses (HuSaVs), which cause acute gastroenteritis, are highly diverse. Fifteen HuSaV genotype strains (GI.1-7, GII.1-5, -8, and GV.1-2) were efficiently propagated in the human duodenum-derived cell line HuTu80 when supplemented with conjugated bile acid (T. Oka, T.-C. Li, K. Yonemitsu, Y. Ami, et al., J Virol 98:e00639-24, 2024, https://doi.org/10.1128/jvi.00639-24). However, the host cellular factors involved in HuSaV infection and propagation remain unidentified. Using a knockout approach, we newly identified and confirmed that CD36 is a critical cellular protein for the propagation of all 15 HuSaV genotypes tested in HuTu80 cells. When the CD36 gene was re-expressed in CD36 gene knockout HuTu80 cells, all HuSaV genotype strains, except for GI.6 and GII.3, recovered their viJournal of virologyCD36 is required for human sapovirus propagation.PMC12646008JP23fk0108683JP23K21362JP24K13428JPMH22KA1001JPMH25KA1001JP20K08320Takagi HOka TOkemoto-Nakamura YfalseCD36 is required for human sapovirus propagation.Human sapoviruses (HuSaVs), which cause acute gastroenteritis, are highly diverse. Fifteen HuSaV genotype strains (GI.1-7, GII.1-5, -8, and GV.1-2) were efficiently propagated in the human duodenum-derived cell line HuTu80 when supplemented with conjugated bile acid (T. Oka, T.-C. Li, K. Yonemitsu, Y. Ami, et al., J Virol 98:e00639-24, 2024, https://doi.org/10.1128/jvi.00639-24). However, the host cellular factors involved in HuSaV infection and propagation remain unidentified. Using a knockout approach, we newly identified and confirmed that CD36 is a critical cellular protein for the propagation of all 15 HuSaV genotypes tested in HuTu80 cells. When the CD36 gene was re-expressed in CD36 gene knockout HuTu80 cells, all HuSaV genotype strains, except for GI.6 and GII.3, recovered their vi2025-01-01T00:00:00Z2025 Nov2026-06-05T19:07:39.388Z2026-05-20T03:14:51.077ZS-EPMC126460084119081310.1128/jvi.01325-25