biostudies-literatureUnknown16Sun G<h4>Background</h4>Emerging evidence indicates a link between gut dysbiosis and allergic rhinitis (AR) pathogenesis. Nevertheless, the mechanistic role of gut microbiota in AR progression requires further characterization. To address this, we employed an integrated multi-omics strategy to delineate gut microbial composition and metabolic signatures in AR patients.<h4>Methods</h4>Fecal specimens from 23 AR patients and 15 matched healthy controls (total <i>n</i> = 38) were subjected to 16S rRNA gene sequencing to assess bacterial community structure, alongside untargeted metabolomic profiling of microbial metabolites. Spearman's rank correlation analysis was applied to evaluate microbiota-metabolite interactions.<h4>Results</h4>Allergic rhinitis patients exhibited altered gut microbial community structure (beta diversity, <i>P</i> < 0.05) with depletion of SCFA-producing genera such as <i>Faecalibacterium</i> and enrichment of pro-inflammatory taxa like <i>Fusobacterium</i>. Metabolomic profiling identified significant disturbances in pathways including pantothenate and CoA biosynthesis, glycolysis, and pyruvate metabolism. Key discriminatory metabolites included maltol and 4-coumaric acid. Integrative analysis revealed significant correlations between specific bacteria and metabolites, such as <i>Faecalibacterium</i> with D-phenyllactic acid (ρ = 0.515, <i>q</i> = 0.046).<h4>Conclusion</h4>Our findings demonstrate that AR is associated with gut dysbiosis and metabolic dysfunction, highlighting the role of microbial-derived metabolites in immune regulation via the gut-nose axis. These insights support the potential for microbiota-targeted therapeutic strategies in AR management.Frontiers in microbiology1652915https://www.ebi.ac.uk/biostudies/studies/S-EPMC12646992biostudies-literatureIntegrated gut microbiome and metabolomics analysis reveals microbial-metabolic cross-talk in allergic rhinitis.PMC12646992Zhao SGuan WChai RHuang HZhang HHou DXu CZhang MSun GWang XfalseIntegrated gut microbiome and metabolomics analysis reveals microbial-metabolic cross-talk in allergic rhinitis.<h4>Background</h4>Emerging evidence indicates a link between gut dysbiosis and allergic rhinitis (AR) pathogenesis. Nevertheless, the mechanistic role of gut microbiota in AR progression requires further characterization. To address this, we employed an integrated multi-omics strategy to delineate gut microbial composition and metabolic signatures in AR patients.<h4>Methods</h4>Fecal specimens from 23 AR patients and 15 matched healthy controls (total <i>n</i> = 38) were subjected to 16S rRNA gene sequencing to assess bacterial community structure, alongside untargeted metabolomic profiling of microbial metabolites. Spearman's rank correlation analysis was applied to evaluate microbiota-metabolite interactions.<h4>Results</h4>Allergic rhinitis patients exhibited altered gut microbial community structure (beta diversity, <i>P</i> < 0.05) with depletion of SCFA-producing genera such as <i>Faecalibacterium</i> and enrichment of pro-inflammatory taxa like <i>Fusobacterium</i>. Metabolomic profiling identified significant disturbances in pathways including pantothenate and CoA biosynthesis, glycolysis, and pyruvate metabolism. Key discriminatory metabolites included maltol and 4-coumaric acid. Integrative analysis revealed significant correlations between specific bacteria and metabolites, such as <i>Faecalibacterium</i> with D-phenyllactic acid (ρ = 0.515, <i>q</i> = 0.046).<h4>Conclusion</h4>Our findings demonstrate that AR is associated with gut dysbiosis and metabolic dysfunction, highlighting the role of microbial-derived metabolites in immune regulation via the gut-nose axis. These insights support the potential for microbiota-targeted therapeutic strategies in AR management.2025-01-01T00:00:00Z20252026-06-05T16:49:55.863Z2026-05-19T03:11:47.333ZS-EPMC126469924131150210.3389/fmicb.2025.1652915