biostudies-literatureUnknown14(11)Yingkajorn M<b>Background/Objectives</b>: <i>Bacteroides</i> species are key members of the human gut microbiota but can act as opportunistic pathogens. This study investigated the genomic features of clinical <i>Bacteroides</i> isolates from southern Thailand. <b>Methods</b>: Sixteen isolates were collected from body fluids, tissues, and pus at Songklanagarind Hospital (2022-2024). Whole-genome sequencing was performed on the BGI platform, followed by genome assembly, annotation, average nucleotide identity (ANI), pairwise single-nucleotide polymorphism (SNP) analysis, antimicrobial resistance (AMR) gene profiling, plasmid prediction, virulence screening, and phylogenetic analysis. <b>Results</b>: ANI and SNP analysis revealed two clusters: one comprising <i>B. ovatus</i>, <i>B. intestinigallinarum</Antibiotics (Basel, Switzerland)1143https://www.ebi.ac.uk/biostudies/studies/S-EPMC12649151biostudies-literaturePhylogenomics and Antimicrobial Resistance of Clinical <i>Bacteroides</i> Isolates from a Tertiary Hospital in Southern Thailand.PMC12649151Pomwised RWonglapsuwan MDuangsi-Ngoen WKlaysubun CSingkhamanan KYaikhan TYingkajorn MChusri SDechathai TSurachat KfalsePhylogenomics and Antimicrobial Resistance of Clinical <i>Bacteroides</i> Isolates from a Tertiary Hospital in Southern Thailand.<b>Background/Objectives</b>: <i>Bacteroides</i> species are key members of the human gut microbiota but can act as opportunistic pathogens. This study investigated the genomic features of clinical <i>Bacteroides</i> isolates from southern Thailand. <b>Methods</b>: Sixteen isolates were collected from body fluids, tissues, and pus at Songklanagarind Hospital (2022-2024). Whole-genome sequencing was performed on the BGI platform, followed by genome assembly, annotation, average nucleotide identity (ANI), pairwise single-nucleotide polymorphism (SNP) analysis, antimicrobial resistance (AMR) gene profiling, plasmid prediction, virulence screening, and phylogenetic analysis. <b>Results</b>: ANI and SNP analysis revealed two clusters: one comprising <i>B. ovatus</i>, <i>B. intestinigallinarum</2025-01-01T00:00:00Z2025 Nov2026-05-19T03:14:57.22Z2026-05-19T03:11:11.547ZS-EPMC126491514130163810.3390/antibiotics14111143