biostudies-literatureZhu JHainan Province Science and Technology Special FundsEarmarked fundNational Natural Science Foundation of ChinaChina Agriculture Research System of MOF and MARA, the Central Public-interest Scientific Institution Basal Research FundKey Laboratory of Tropical Marine Ecosystem and Bioresource, Ministry of Natural ResourcesTaishan Scholars Program of Shandong Province3249https://www.ebi.ac.uk/biostudies/studies/S-EPMC12649247biostudies-literatureUnknown15(22)To evaluate the aquaculture potential of <i>Amphioctopus kagoshimensis</i>, we investigated the reproductive biology, embryonic development, and early paralarval morphology of <i>Amphioctopus kagoshimensis</i> under controlled laboratory conditions. Each adult specimen collected from the coastal waters of Fujian Province spawned approximately 4000-5000 eggs (mean ± SD: 4375 ± 478 eggs), with an overall hatching rate of 75% ± 10% (<i>n</i> = 2). Embryonic development lasted approximately 30 days at 22.0-24.5 °C and followed a classical 20-stage pattern. Hatchlings measured an average mantle length of 1.4 ± 0.1 mm and exhibited a merobenthic strategy, characterized by planktonic paralarvae with progressive morphological differentiation. The chromatophores appeared progressively on the head, Animals : an open access journal from MDPIFirst Observation of Embryonic Development and Paralarvae of &lt;i&gt;Amphioctopus kagoshimensis&lt;/i&gt;.PMC126492472023TD30CARS-49No. 2024QN0332303036ZDYF2024GXJS308 and ZDYF2022GXJS347No. tsqn202507298Zhang TZhu JChang QBian LYu JChen SfalseFirst Observation of Embryonic Development and Paralarvae of &lt;i&gt;Amphioctopus kagoshimensis&lt;/i&gt;.To evaluate the aquaculture potential of <i>Amphioctopus kagoshimensis</i>, we investigated the reproductive biology, embryonic development, and early paralarval morphology of <i>Amphioctopus kagoshimensis</i> under controlled laboratory conditions. Each adult specimen collected from the coastal waters of Fujian Province spawned approximately 4000-5000 eggs (mean ± SD: 4375 ± 478 eggs), with an overall hatching rate of 75% ± 10% (<i>n</i> = 2). Embryonic development lasted approximately 30 days at 22.0-24.5 °C and followed a classical 20-stage pattern. Hatchlings measured an average mantle length of 1.4 ± 0.1 mm and exhibited a merobenthic strategy, characterized by planktonic paralarvae with progressive morphological differentiation. The chromatophores appeared progressively on the head, 2025-01-01T00:00:00Z2025 Nov2026-05-20T03:19:31.516Z2026-05-20T03:13:33.068ZS-EPMC126492474130196010.3390/ani15223249