biostudies-literatureXiao XJilin Provincial Natural Science FoundationNational Natural Science Foundation of China General Program1314https://www.ebi.ac.uk/biostudies/studies/S-EPMC12649525biostudies-literatureUnknown14(11)In this study, we constructed an extraction process for bovine lung peptide-1 (BLP-1) derived from bovine lung tissue utilizing single-factor optimization combined with response surface methodology. We systematically analyzed its antioxidant activity, biological safety, and therapeutic mechanisms against alcoholic liver disease (ALD). In vitro experiments demonstrated that BLP-1 exhibits excellent scavenging activity against various free radicals, while exhibiting no significant cytotoxicity or hemolytic activity. In a model of H<sub>2</sub>O<sub>2</sub>-induced oxidative damage in HepG2 cells, BLP-1 significantly alleviated oxidative stress injury by upregulating the activities of intracellular antioxidant enzymes. Animal experiments further confirmed that BLP-1 significantly reduced seruAntioxidants (Basel, Switzerland)Extraction, Purification and Identification of Bovine Lung Peptides and Its Antioxidant Effects on H&lt;sub&gt;2&lt;/sub&gt;O&lt;sub&gt;2&lt;/sub&gt;-Induced HepG2 Cells and Mice with Alcoholic Liver Injury.PMC126495253227305820220101286JCSu TLi YFang JHao LZhang XWang DXiao XZheng SLv QfalseExtraction, Purification and Identification of Bovine Lung Peptides and Its Antioxidant Effects on H&lt;sub&gt;2&lt;/sub&gt;O&lt;sub&gt;2&lt;/sub&gt;-Induced HepG2 Cells and Mice with Alcoholic Liver Injury.In this study, we constructed an extraction process for bovine lung peptide-1 (BLP-1) derived from bovine lung tissue utilizing single-factor optimization combined with response surface methodology. We systematically analyzed its antioxidant activity, biological safety, and therapeutic mechanisms against alcoholic liver disease (ALD). In vitro experiments demonstrated that BLP-1 exhibits excellent scavenging activity against various free radicals, while exhibiting no significant cytotoxicity or hemolytic activity. In a model of H<sub>2</sub>O<sub>2</sub>-induced oxidative damage in HepG2 cells, BLP-1 significantly alleviated oxidative stress injury by upregulating the activities of intracellular antioxidant enzymes. Animal experiments further confirmed that BLP-1 significantly reduced seru2025-01-01T00:00:00Z2025 Oct2026-05-19T03:18:56.709Z2026-05-19T03:11:32.916ZS-EPMC126495254130047110.3390/antiox14111314