biostudies-literatureCai Gthe Natural Science Foundation of Rizhaothe Doctor Startup Foundation of Jining Medical Universitythe Natural Science Foundation of Shandong1566https://www.ebi.ac.uk/biostudies/studies/S-EPMC12650281biostudies-literatureUnknown14(11)Soil salinization significantly limits plant growth and agricultural productivity, with MYB transcription factors playing crucial roles in mediating plant responses to salt stress. In this study, a novel R2R3-MYB transcription factor gene, <i>SlMYB306-like</i>, was isolated from tomato. Phylogenetic comparison indicated that SlMYB306-like shared the highest sequence homology with potato StMYB306-like. Subcellular localization assays demonstrated nuclear localization of SlMYB306-like protein, while yeast transactivation assays confirmed its function as a transcriptional activator. Expression profiling showed that <i>SlMYB306-like</i> was inducible by NaCl and abscisic acid (ABA) treatments. In addition, functional characterization via the overexpression of <i>SlMYB306-like</i> in <i>ArabidoBiologyCharacterization of a Novel Tomato R2R3-MYB Transcription Factor Gene, <i>SlMYB306-like</i>, Conferring Salt Tolerance in <i>Arabidopsis</i>.PMC12650281ZR2022QC240600940001RZ2021ZR19He JGao JWang WWang GCai GChen TWang LYin ZfalseCharacterization of a Novel Tomato R2R3-MYB Transcription Factor Gene, <i>SlMYB306-like</i>, Conferring Salt Tolerance in <i>Arabidopsis</i>.Soil salinization significantly limits plant growth and agricultural productivity, with MYB transcription factors playing crucial roles in mediating plant responses to salt stress. In this study, a novel R2R3-MYB transcription factor gene, <i>SlMYB306-like</i>, was isolated from tomato. Phylogenetic comparison indicated that SlMYB306-like shared the highest sequence homology with potato StMYB306-like. Subcellular localization assays demonstrated nuclear localization of SlMYB306-like protein, while yeast transactivation assays confirmed its function as a transcriptional activator. Expression profiling showed that <i>SlMYB306-like</i> was inducible by NaCl and abscisic acid (ABA) treatments. In addition, functional characterization via the overexpression of <i>SlMYB306-like</i> in <i>Arabido2025-01-01T00:00:00Z2025 Nov2026-05-19T03:17:11.678Z2026-05-19T03:11:36.554ZS-EPMC126502814130035610.3390/biology14111566