biostudies-literatureLuo YGuangdong Basic and Applied Basic Research FoundationGuangdong Academy of Agricultural Sciences Talent Introduction Project for 2022Guangzhou Municipal Financial Grant Project for Agriculture and Rural Affairs1520https://www.ebi.ac.uk/biostudies/studies/S-EPMC12650335biostudies-literatureUnknown15(11)<i>Anubias</i> (Araceae) is a globally important group of ornamental aquatic plants. However, when temperatures drop to 10 °C, most species suffer obvious frostbite from cold stress, restricting winter cultivation and broader application. This study focused on two <i>Anubias</i> genotypes with distinct cold tolerance, adopting an integrated approach combining phenotypic, physiological, metabolomic, and transcriptomic analyses to reveal the mechanisms underlying their differential cold tolerance. Under 10 °C cold stress, compared with normal temperatures, the leaves of cold-tolerant <i>Anubias</i> sp. 'Long Leaf' (Jian) showed no significant frostbite, while cold-sensitive <i>Anubias barteri</i> var. <i>nana</i> 'Coin Leaf' (Jin) had clear frost damage. Both genotypes exhibited increased leBiomoleculesIntegrative Physiological, Metabolomic and Transcriptomic Analyses Uncover the Mechanisms Underlying Differential Responses of Two &lt;i&gt;Anubias&lt;/i&gt; Genotypes to Low-Temperature Stress.PMC12650335R2022YJ-YB30232023A151514002125100001Yang ZHuang SChen JZhang JLuo YZhou YWei LLiu WfalseIntegrative Physiological, Metabolomic and Transcriptomic Analyses Uncover the Mechanisms Underlying Differential Responses of Two &lt;i&gt;Anubias&lt;/i&gt; Genotypes to Low-Temperature Stress.<i>Anubias</i> (Araceae) is a globally important group of ornamental aquatic plants. However, when temperatures drop to 10 °C, most species suffer obvious frostbite from cold stress, restricting winter cultivation and broader application. This study focused on two <i>Anubias</i> genotypes with distinct cold tolerance, adopting an integrated approach combining phenotypic, physiological, metabolomic, and transcriptomic analyses to reveal the mechanisms underlying their differential cold tolerance. Under 10 °C cold stress, compared with normal temperatures, the leaves of cold-tolerant <i>Anubias</i> sp. 'Long Leaf' (Jian) showed no significant frostbite, while cold-sensitive <i>Anubias barteri</i> var. <i>nana</i> 'Coin Leaf' (Jin) had clear frost damage. Both genotypes exhibited increased le2025-01-01T00:00:00Z2025 Oct2026-05-20T03:20:14.739Z2026-05-20T03:13:50.43ZS-EPMC126503354130143910.3390/biom15111520