{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Nagaoka K"],"funding":["JSPS KAKENHI","Japan Agency for Medical Research and Development (AMED)"],"pagination":["1789"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12650950"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["14(22)"],"pubmed_abstract":["Targeting mutation-derived neoantigens is a promising strategy for personalized immunotherapies. However, identifying true neoantigens and cognate T cell receptors (TCRs) remains challenging because computational prediction of neoantigen peptides is uncertain and most tumor-infiltrating lymphocytes are bystanders rather than tumor-reactive, necessitating functional validation. Here, we developed NeoPAIR-T (Neoantigen-TCR Pairing Assay using reporter T cells), a functional assay based on co-culture of TCR-T reporter cells and autologous antigen-presenting cells (APCs) to screen neoantigen-TCR pairs. Reporter T cells are Jurkat-derived cells engineered to express a luciferase/eGFP dual reporter, providing quantitative readouts of TCR activation, while APCs are immortalized autologous cells t"],"journal":["Cells"],"pubmed_title":["NeoPAIR-T: Functional Mapping of Neoantigen-TCR Pairs Using a CRISPR-Engineered Jurkat Reporter System."],"pmcid":["PMC12650950"],"funding_grant_id":["24ama221321h0002","23H02762"],"pubmed_authors":["Nagaoka K","Kobayashi Y","Kakimi K"],"additional_accession":[]},"is_claimable":false,"name":"NeoPAIR-T: Functional Mapping of Neoantigen-TCR Pairs Using a CRISPR-Engineered Jurkat Reporter System.","description":"Targeting mutation-derived neoantigens is a promising strategy for personalized immunotherapies. However, identifying true neoantigens and cognate T cell receptors (TCRs) remains challenging because computational prediction of neoantigen peptides is uncertain and most tumor-infiltrating lymphocytes are bystanders rather than tumor-reactive, necessitating functional validation. Here, we developed NeoPAIR-T (Neoantigen-TCR Pairing Assay using reporter T cells), a functional assay based on co-culture of TCR-T reporter cells and autologous antigen-presenting cells (APCs) to screen neoantigen-TCR pairs. Reporter T cells are Jurkat-derived cells engineered to express a luciferase/eGFP dual reporter, providing quantitative readouts of TCR activation, while APCs are immortalized autologous cells t","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Nov","modification":"2026-05-20T03:20:19.081Z","creation":"2026-05-20T03:13:48.265Z"},"accession":"S-EPMC12650950","cross_references":{"pubmed":["41294842"],"doi":["10.3390/cells14221789"]}}