{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Tran NK"],"funding":["Ministry of Science and Technology"],"pagination":["906"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12651245"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["47(11)"],"pubmed_abstract":["Foodborne illnesses associated with <i>Bacillus cereus</i> represent a persistent public health concern. In this study, we described the isolation and characterization of a novel bacteriophage, ΦBc24, from mud samples, which showed lytic activity against foodborne pathogen <i>B. cereus</i>. Transmission electron microscopy revealed that ΦBc24 exhibited a myovirus morphotype. Biological assays demonstrated that its narrow host range was restricted to <i>B. cereus</i> strains and efficient lytic activity, characterized by a latent period of 10 min and a burst size of 40 PFU per infected cell. The phage exhibited high physicochemical stability, tolerating pH values of 2-12, temperatures of 4-50 °C, salinity up to 1 M NaCl, and ultraviolet exposure, while effectively suppressing host bacterial"],"journal":["Current issues in molecular biology"],"pubmed_title":["Genomic and Biological Insights of Bacteriophage ΦBc24 Targeting &lt;i&gt;Bacillus cereus&lt;/i&gt;."],"pmcid":["PMC12651245"],"funding_grant_id":["Grant No. NVQG 2022/ĐT.06"],"pubmed_authors":["Quyen DV","Lanh PT","Hien VT","Phuoc MH","Tran NK","Trinh Thu T","Duy ND"],"additional_accession":[]},"is_claimable":false,"name":"Genomic and Biological Insights of Bacteriophage ΦBc24 Targeting &lt;i&gt;Bacillus cereus&lt;/i&gt;.","description":"Foodborne illnesses associated with <i>Bacillus cereus</i> represent a persistent public health concern. In this study, we described the isolation and characterization of a novel bacteriophage, ΦBc24, from mud samples, which showed lytic activity against foodborne pathogen <i>B. cereus</i>. Transmission electron microscopy revealed that ΦBc24 exhibited a myovirus morphotype. Biological assays demonstrated that its narrow host range was restricted to <i>B. cereus</i> strains and efficient lytic activity, characterized by a latent period of 10 min and a burst size of 40 PFU per infected cell. The phage exhibited high physicochemical stability, tolerating pH values of 2-12, temperatures of 4-50 °C, salinity up to 1 M NaCl, and ultraviolet exposure, while effectively suppressing host bacterial","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Oct","modification":"2026-06-02T03:17:39.441Z","creation":"2026-06-02T03:09:46.737Z"},"accession":"S-EPMC12651245","cross_references":{"pubmed":["41296410"],"doi":["10.3390/cimb47110906"]}}