biostudies-literatureTran NKMinistry of Science and Technology906https://www.ebi.ac.uk/biostudies/studies/S-EPMC12651245biostudies-literatureUnknown47(11)Foodborne illnesses associated with <i>Bacillus cereus</i> represent a persistent public health concern. In this study, we described the isolation and characterization of a novel bacteriophage, ΦBc24, from mud samples, which showed lytic activity against foodborne pathogen <i>B. cereus</i>. Transmission electron microscopy revealed that ΦBc24 exhibited a myovirus morphotype. Biological assays demonstrated that its narrow host range was restricted to <i>B. cereus</i> strains and efficient lytic activity, characterized by a latent period of 10 min and a burst size of 40 PFU per infected cell. The phage exhibited high physicochemical stability, tolerating pH values of 2-12, temperatures of 4-50 °C, salinity up to 1 M NaCl, and ultraviolet exposure, while effectively suppressing host bacterialCurrent issues in molecular biologyGenomic and Biological Insights of Bacteriophage ΦBc24 Targeting &lt;i&gt;Bacillus cereus&lt;/i&gt;.PMC12651245Grant No. NVQG 2022/ĐT.06Quyen DVLanh PTHien VTPhuoc MHTran NKTrinh Thu TDuy NDfalseGenomic and Biological Insights of Bacteriophage ΦBc24 Targeting &lt;i&gt;Bacillus cereus&lt;/i&gt;.Foodborne illnesses associated with <i>Bacillus cereus</i> represent a persistent public health concern. In this study, we described the isolation and characterization of a novel bacteriophage, ΦBc24, from mud samples, which showed lytic activity against foodborne pathogen <i>B. cereus</i>. Transmission electron microscopy revealed that ΦBc24 exhibited a myovirus morphotype. Biological assays demonstrated that its narrow host range was restricted to <i>B. cereus</i> strains and efficient lytic activity, characterized by a latent period of 10 min and a burst size of 40 PFU per infected cell. The phage exhibited high physicochemical stability, tolerating pH values of 2-12, temperatures of 4-50 °C, salinity up to 1 M NaCl, and ultraviolet exposure, while effectively suppressing host bacterial2025-01-01T00:00:00Z2025 Oct2026-06-02T03:17:39.441Z2026-06-02T03:09:46.737ZS-EPMC126512454129641010.3390/cimb47110906