{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Murphy KR"],"funding":["Grayton Friedlander Memorial Fund","National Institute of Health","NCI NIH HHS","NIH HHS","Virginia-Maryland College of Veterinary Medicine Internal Research Competition"],"pagination":["1782"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12651280"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["14(22)"],"pubmed_abstract":["High-frequency irreversible electroporation (H-FIRE) is a nonthermal tumor ablation technique that disrupts the blood-brain barrier (BBB) in a focal and reversible manner. However, the mechanisms underlying this disruption remain poorly understood, particularly the role of small tumor-derived extracellular vesicles (sTDEVs) released from ablated tumor cells. In this study, we investigate the proteomic and functional alterations of sTDEVs released from F98 glioma and LL/2 Lewis lung carcinoma cells following H-FIRE ablation. Mass spectrometry analysis revealed 108 unique proteins in sTDEVs derived from ablative doses of H-FIRE, which are capable of disrupting the BBB in an in vitro model. Proteomic analysis of TDEVs highlights key changes in pathways related to integrin signaling, Platelet-"],"journal":["Cells"],"pubmed_title":["High-Frequency Irreversible Electroporation Alters Proteomic Profiles and Tropism of Small Tumor-Derived Extracellular Vesicles to Promote Immune Cell Infiltration."],"pmcid":["PMC12651280"],"funding_grant_id":["S10 OD020111","N/A","R01CA213423","1S10OD020111-01","R01 CA213423"],"pubmed_authors":["Aycock KN","Bracha S","Marsh S","Yang L","Murphy KR","Rossmeisl JH","Dervisis NG","Selmek A","Gourdie R","Hinckley J","Davalos RV"],"additional_accession":[]},"is_claimable":false,"name":"High-Frequency Irreversible Electroporation Alters Proteomic Profiles and Tropism of Small Tumor-Derived Extracellular Vesicles to Promote Immune Cell Infiltration.","description":"High-frequency irreversible electroporation (H-FIRE) is a nonthermal tumor ablation technique that disrupts the blood-brain barrier (BBB) in a focal and reversible manner. However, the mechanisms underlying this disruption remain poorly understood, particularly the role of small tumor-derived extracellular vesicles (sTDEVs) released from ablated tumor cells. In this study, we investigate the proteomic and functional alterations of sTDEVs released from F98 glioma and LL/2 Lewis lung carcinoma cells following H-FIRE ablation. Mass spectrometry analysis revealed 108 unique proteins in sTDEVs derived from ablative doses of H-FIRE, which are capable of disrupting the BBB in an in vitro model. Proteomic analysis of TDEVs highlights key changes in pathways related to integrin signaling, Platelet-","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Nov","modification":"2026-07-04T03:13:23.554Z","creation":"2026-07-04T03:11:30.943Z"},"accession":"S-EPMC12651280","cross_references":{"pubmed":["41294835"],"doi":["10.3390/cells14221782"]}}