biostudies-literatureDong YNational Natural Science Foundation of China3926https://www.ebi.ac.uk/biostudies/studies/S-EPMC12651533biostudies-literatureUnknown14(22)The objective of this study was to evaluate the structural, functional and morphological characterizations of ultrasonic-assisted extraction of crude prolamin from amaranth grain (<i>Amaranthus hypochondriacus</i> L.). A Box-Behnken design of response surface methodology (RSM) was employed to optimize the extraction parameters. The optimal extraction parameters included a solid/solvent ratio of 1:9 (g:mL), with 50% ethanol solution at 75 °C. Regarding the physicochemical characteristics, amaranth crude prolamin (ACP) possessed more orderly secondary structures (the proportions of α-helix and β-sheet were 43.65% and 25.52%, respectively), which is favorable for improving the object-holding capacity, such as higher oil- and water-holding capacity. The higher surface hydrophobicity of ACP wasFoods (Basel, Switzerland)Extraction and Functional Properties of Crude Prolamin from Amaranth.PMC1265153351703100Dong YHu XHe LWang YfalseExtraction and Functional Properties of Crude Prolamin from Amaranth.The objective of this study was to evaluate the structural, functional and morphological characterizations of ultrasonic-assisted extraction of crude prolamin from amaranth grain (<i>Amaranthus hypochondriacus</i> L.). A Box-Behnken design of response surface methodology (RSM) was employed to optimize the extraction parameters. The optimal extraction parameters included a solid/solvent ratio of 1:9 (g:mL), with 50% ethanol solution at 75 °C. Regarding the physicochemical characteristics, amaranth crude prolamin (ACP) possessed more orderly secondary structures (the proportions of α-helix and β-sheet were 43.65% and 25.52%, respectively), which is favorable for improving the object-holding capacity, such as higher oil- and water-holding capacity. The higher surface hydrophobicity of ACP was2025-01-01T00:00:00Z2025 Nov2026-05-19T03:17:03.736Z2026-05-19T03:11:35.85ZS-EPMC126515334130008410.3390/foods14223926