{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Wiegand A"],"funding":["Deutsche Forschungsgemeinschaft","German Federal Ministry of Education and Research (BMBF)"],"pagination":["10840"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12652996"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["26(22)"],"pubmed_abstract":["Alcohol use disorder (AUD) is associated with widespread epigenetic alterations, including changes in DNA methylation (DNAm). This multi-cohort study validated and extended previous findings on DNAm of <i>HECW2</i> and <i>GDAP1</i> in AUD, assessed sex differences, and explored DNAm in blood and brain tissue in humans and rats. DNAm was measured via pyrosequencing in human blood (N<sub>Ctrl</sub> = 341, N<sub>AUD</sub> = 258), postmortem frontal cortex (Brodmann area 9; discovery cohort: N<sub>Ctrl</sub> = 10, N<sub>AUD</sub> = 13, replication cohort: N<sub>Ctrl</sub> = 64, N<sub>AUD</sub> = 55) and rat blood and medial prefrontal cortex (N<sub>Ctrl</sub> = 16, N<sub>AUD</sub> = 15). Gene expression was assessed in human postmortem brain by quantitative real-time PCR. AUD-associated DNAm d"],"journal":["International journal of molecular sciences"],"pubmed_title":["Validation of &lt;i&gt;GDAP1&lt;/i&gt; and &lt;i&gt;HECW2&lt;/i&gt; as Epigenetic Markers of Alcohol Use Disorder in Blood and Brain."],"pmcid":["PMC12652996"],"funding_grant_id":["NI13332/13-1","01ZX01909","TRR265"],"pubmed_authors":["Koller G","Friske M","Zill P","Hansson AC","Nieratschker V","Wiegand A","Edelmann S","Sommer WH","Bakalkin G","Fischer L","Bender A"],"additional_accession":[]},"is_claimable":false,"name":"Validation of &lt;i&gt;GDAP1&lt;/i&gt; and &lt;i&gt;HECW2&lt;/i&gt; as Epigenetic Markers of Alcohol Use Disorder in Blood and Brain.","description":"Alcohol use disorder (AUD) is associated with widespread epigenetic alterations, including changes in DNA methylation (DNAm). This multi-cohort study validated and extended previous findings on DNAm of <i>HECW2</i> and <i>GDAP1</i> in AUD, assessed sex differences, and explored DNAm in blood and brain tissue in humans and rats. DNAm was measured via pyrosequencing in human blood (N<sub>Ctrl</sub> = 341, N<sub>AUD</sub> = 258), postmortem frontal cortex (Brodmann area 9; discovery cohort: N<sub>Ctrl</sub> = 10, N<sub>AUD</sub> = 13, replication cohort: N<sub>Ctrl</sub> = 64, N<sub>AUD</sub> = 55) and rat blood and medial prefrontal cortex (N<sub>Ctrl</sub> = 16, N<sub>AUD</sub> = 15). Gene expression was assessed in human postmortem brain by quantitative real-time PCR. AUD-associated DNAm d","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Nov","modification":"2026-05-19T03:14:02.324Z","creation":"2026-05-19T03:10:38.449Z"},"accession":"S-EPMC12652996","cross_references":{"pubmed":["41303328"],"doi":["10.3390/ijms262210840"]}}