biostudies-literatureLin TKMinistry of Science and Technology, TaiwanChina Medical University, TaiwanNational Science and Technology Council, TaiwanHualien Tzu Chi Hospital1707https://www.ebi.ac.uk/biostudies/studies/S-EPMC12653036biostudies-literatureUnknown15(11)Contact dermatitis is a common inflammatory skin disorder characterized by erythematous and pruritic lesions caused by irritant exposure. <i>Ohwia caudata</i>, a traditional medicinal plant, possesses anti-inflammatory and antioxidant properties, but its dermatoprotective potential remains unclear. To investigate the protective effects and mechanisms of <i>Ohwia caudata</i> leaves' hydroethanolic extract in a murine model of TPA-induced contact dermatitis. Major phytochemicals in <i>Ohwia caudata</i> extract were identified by HPLC-MS. SKH1/J hairless mice were topically treated with <i>Ohwia caudata</i> extract following TPA stimulation. Skin barrier function was assessed by transepidermal water loss and hydration. Inflammatory (IL-6, TNF-α, TGF-β), antioxidant (SIRT1, Nrf2, HO-1), and ERLife (Basel, Switzerland)Protective Anti-Inflammatory and Antioxidant Mechanisms of &lt;i&gt;Ohwia caudata&lt;/i&gt; Leaf Hydroethanolic Extract in a Dermatitis Mouse Model.PMC12653036noKuo WWTsai BCHo TJHsieh DJHuang CYLin TKLin SZTsai CLKao SWKuo CHfalseProtective Anti-Inflammatory and Antioxidant Mechanisms of &lt;i&gt;Ohwia caudata&lt;/i&gt; Leaf Hydroethanolic Extract in a Dermatitis Mouse Model.Contact dermatitis is a common inflammatory skin disorder characterized by erythematous and pruritic lesions caused by irritant exposure. <i>Ohwia caudata</i>, a traditional medicinal plant, possesses anti-inflammatory and antioxidant properties, but its dermatoprotective potential remains unclear. To investigate the protective effects and mechanisms of <i>Ohwia caudata</i> leaves' hydroethanolic extract in a murine model of TPA-induced contact dermatitis. Major phytochemicals in <i>Ohwia caudata</i> extract were identified by HPLC-MS. SKH1/J hairless mice were topically treated with <i>Ohwia caudata</i> extract following TPA stimulation. Skin barrier function was assessed by transepidermal water loss and hydration. Inflammatory (IL-6, TNF-α, TGF-β), antioxidant (SIRT1, Nrf2, HO-1), and ER2025-01-01T00:00:00Z2025 Nov2026-05-20T03:16:55.776Z2026-05-20T03:08:55.061ZS-EPMC126530364130213210.3390/life15111707