biostudies-literatureAguila-Torres PRoyal Society Research Fellows Enhanced grant (M.H.)Millennium Science Initiative Program (M.G.)Universidad Austral (P.A-T)Royal Society Dorothy Hodgkin Research Fellowship (M.H.)2519https://www.ebi.ac.uk/biostudies/studies/S-EPMC12654796biostudies-literatureUnknown13(11)Osorno volcano (41.1° S, 72° W) is located in the Andean Southern Volcanic Zone. The volcano lies within a national park as part of the protected areas system. This setting provides an opportunity to compare soil microbial communities between sectors with (H) and without (NI) anthropogenic activities within a volcanic territory. To do so, we selected one of the most visited volcanoes in Chilean Patagonia to examine composition, diversity (taxonomic and phylogenetic), and co-presence and mutual exclusion interaction networks between members of volcanic soil bacterial communities. Soil DNA was extracted, and the 16S rRNA gene was analyzed by high-throughput DNA sequencing, followed by taxonomic identification. The most prevalent phylum across all sites (H and NI) was <i>Pseudomonadota</i>, fMicroorganismsCharacterization of Bacterial Communities in Volcanic Soil from Northern Patagonian Area of Chile.PMC12654796DHF\R1\2110762025RF\ERE\210050ICN2021_044Palma DEMiranda RMRojas LAHernandez MGonzalez MGonzalez-Stegmaier RAguila-Torres PAguado-Norese CMaldonado JEMellado MfalseCharacterization of Bacterial Communities in Volcanic Soil from Northern Patagonian Area of Chile.Osorno volcano (41.1° S, 72° W) is located in the Andean Southern Volcanic Zone. The volcano lies within a national park as part of the protected areas system. This setting provides an opportunity to compare soil microbial communities between sectors with (H) and without (NI) anthropogenic activities within a volcanic territory. To do so, we selected one of the most visited volcanoes in Chilean Patagonia to examine composition, diversity (taxonomic and phylogenetic), and co-presence and mutual exclusion interaction networks between members of volcanic soil bacterial communities. Soil DNA was extracted, and the 16S rRNA gene was analyzed by high-throughput DNA sequencing, followed by taxonomic identification. The most prevalent phylum across all sites (H and NI) was <i>Pseudomonadota</i>, f2025-01-01T00:00:00Z2025 Nov2026-05-20T03:16:32.866Z2026-05-20T03:13:11.035ZS-EPMC126547964130420510.3390/microorganisms13112519