biostudies-literatureGuo HJiangxi Provincial Natural Science FoundationNational Natural Science Foundation of China2430https://www.ebi.ac.uk/biostudies/studies/S-EPMC12654850biostudies-literatureUnknown13(11)Longya Lily (<i>Lilium brownii</i> var. <i>viridulum</i>) bulb rot, a devastating soil-borne disease caused by <i>Fusarium oxysporum</i> f. sp. <i>lilii</i> (Fol L1-1), severely compromises yield and quality of this economically significant crop. In this study, strain R12 was isolated from the rhizosphere soil of asymptomatic Longya lily plants and identified as <i>Bacillus velezensis</i>. The strain significantly disrupted the spore germination and hyphal morphology of Fol L1-1. In pot experiments, R12 not only effectively suppressed disease development but also promoted plant growth, a trait potentially linked to its indole-3-acetic acid (IAA) production capacity. Genomic analysis revealed a 4,015,523 bp circular chromosome (46.42% GC content) harboring gene clusters for the synthesis ofMicroorganismsScreening and Genomic Analysis of &lt;i&gt;Bacillus velezensis&lt;/i&gt; R12 as a Biocontrol Agent Against &lt;i&gt;Fusarium oxysporum&lt;/i&gt; Causing Wilt in Longya Lily (&lt;i&gt;Lilium brownii&lt;/i&gt; var. &lt;i&gt;viridulum&lt;/i&gt;).PMC126548503236008020252BAC24009020242BAB20286;20252BAC240090Zhan MHuang YJi HGuo HZheng QLi QLiu SZhao KZhu DCheng ZfalseScreening and Genomic Analysis of &lt;i&gt;Bacillus velezensis&lt;/i&gt; R12 as a Biocontrol Agent Against &lt;i&gt;Fusarium oxysporum&lt;/i&gt; Causing Wilt in Longya Lily (&lt;i&gt;Lilium brownii&lt;/i&gt; var. &lt;i&gt;viridulum&lt;/i&gt;).Longya Lily (<i>Lilium brownii</i> var. <i>viridulum</i>) bulb rot, a devastating soil-borne disease caused by <i>Fusarium oxysporum</i> f. sp. <i>lilii</i> (Fol L1-1), severely compromises yield and quality of this economically significant crop. In this study, strain R12 was isolated from the rhizosphere soil of asymptomatic Longya lily plants and identified as <i>Bacillus velezensis</i>. The strain significantly disrupted the spore germination and hyphal morphology of Fol L1-1. In pot experiments, R12 not only effectively suppressed disease development but also promoted plant growth, a trait potentially linked to its indole-3-acetic acid (IAA) production capacity. Genomic analysis revealed a 4,015,523 bp circular chromosome (46.42% GC content) harboring gene clusters for the synthesis of2025-01-01T00:00:00Z2025 Oct2026-05-20T03:19:56.89Z2026-05-20T03:13:44.987ZS-EPMC126548504130411510.3390/microorganisms13112430