biostudies-literatureMa YScience & Technology Innovation Program of Yongjiang 2035708https://www.ebi.ac.uk/biostudies/studies/S-EPMC12654858biostudies-literatureUnknown15(11)<b>Background/Objectives</b>: Chronic colitis presents a growing global health burden with rising incidence. This study investigated the ameliorative effect of <i>Dendrobium officinale</i> polysaccharide (DOP) against dextran sulfate sodium (DSS)-induced chronic colitis in mice, specifically examining its dual modulation of gut microbiota and metabolic pathways. <b>Methods</b>: DOP was extracted and purified from <i>Dendrobium officinale</i> stems and leaves. A chronic colitis model was established in male C57BL/6J mice via DSS induction. Eighty-four mice were randomized into seven groups: control, model, low/high-dose leaf-DOP, low/high-dose stem-DOP, and sulfasalazine positive control. We assessed body weight, disease activity index (DAI), colon length, splenic/thymic indices, inflammatoMetabolites&lt;i&gt;Dendrobium officinale&lt;/i&gt; Polysaccharide Relieves the DSS-Induced Chronic Colitis in C57BL/6J Mice and Regulates Colonic Microflora Structure.PMC126548582024Z281Xing JLi JYang YTao WZhang HMa YZhou WYuan Xfalse&lt;i&gt;Dendrobium officinale&lt;/i&gt; Polysaccharide Relieves the DSS-Induced Chronic Colitis in C57BL/6J Mice and Regulates Colonic Microflora Structure.<b>Background/Objectives</b>: Chronic colitis presents a growing global health burden with rising incidence. This study investigated the ameliorative effect of <i>Dendrobium officinale</i> polysaccharide (DOP) against dextran sulfate sodium (DSS)-induced chronic colitis in mice, specifically examining its dual modulation of gut microbiota and metabolic pathways. <b>Methods</b>: DOP was extracted and purified from <i>Dendrobium officinale</i> stems and leaves. A chronic colitis model was established in male C57BL/6J mice via DSS induction. Eighty-four mice were randomized into seven groups: control, model, low/high-dose leaf-DOP, low/high-dose stem-DOP, and sulfasalazine positive control. We assessed body weight, disease activity index (DAI), colon length, splenic/thymic indices, inflammato2025-01-01T00:00:00Z2025 Oct2026-05-20T03:20:46.726Z2026-05-20T03:13:55.244ZS-EPMC126548584129529410.3390/metabo15110708