{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Tang J"],"funding":["National Natural Science Foundation of China","Natural Science Foundation of Heilongjiang Province"],"pagination":["3408"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12656323"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["14(22)"],"pubmed_abstract":["Soil alkalinity severely restricts the cultivation of <i>Lupinus angustifolius</i>, a valuable legume. Wild soybean (<i>Glycine soja</i>) is a leguminous plant with extremely strong alkaline resistance (pH 8.5). Transferring the alkali-tolerant genes from wild soybeans into lupinus can effectively enhance the alkali tolerance. In this study, we combined transcriptome profiling and genetic transformation to elucidate the molecular basis of alkaline stress response in lupinus. RNA-seq analysis of root tips under acid (HCl, pH 4.0) and alkali (NaHCO<sub>3</sub>, pH 8.5) stress revealed 104,353 annotated unigenes, with differential expression patterns highlighting enrichment in cellular component, binding, and catalytic activity categories. KEGG pathway analysis indicated that early responses "],"journal":["Plants (Basel, Switzerland)"],"pubmed_title":["Leveraging Transcriptome Insights and &lt;i&gt;GsHZ4&lt;/i&gt; Gene Expression to Improve Alkaline Tolerance in &lt;i&gt;Lupinus angustifolius&lt;/i&gt;."],"pmcid":["PMC12656323"],"funding_grant_id":["32472750","LH2023C006","32001505"],"pubmed_authors":["Liu X","Zhou M","Gao Q","Cao L","Tang J","Liu Y","Liu M","Zhang J","Feng Y","Du X","Mao H","Wang X"],"additional_accession":[]},"is_claimable":false,"name":"Leveraging Transcriptome Insights and &lt;i&gt;GsHZ4&lt;/i&gt; Gene Expression to Improve Alkaline Tolerance in &lt;i&gt;Lupinus angustifolius&lt;/i&gt;.","description":"Soil alkalinity severely restricts the cultivation of <i>Lupinus angustifolius</i>, a valuable legume. Wild soybean (<i>Glycine soja</i>) is a leguminous plant with extremely strong alkaline resistance (pH 8.5). Transferring the alkali-tolerant genes from wild soybeans into lupinus can effectively enhance the alkali tolerance. In this study, we combined transcriptome profiling and genetic transformation to elucidate the molecular basis of alkaline stress response in lupinus. RNA-seq analysis of root tips under acid (HCl, pH 4.0) and alkali (NaHCO<sub>3</sub>, pH 8.5) stress revealed 104,353 annotated unigenes, with differential expression patterns highlighting enrichment in cellular component, binding, and catalytic activity categories. KEGG pathway analysis indicated that early responses ","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Nov","modification":"2026-07-05T03:18:36.58Z","creation":"2026-07-05T03:12:28.972Z"},"accession":"S-EPMC12656323","cross_references":{"pubmed":["41304559"],"doi":["10.3390/plants14223408"]}}