{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Wang H"],"funding":["Texas Crop Science"],"pagination":["3514"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12656381"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["14(22)"],"pubmed_abstract":["As reported in earlier work, when a pea apyrase, psNTP9 (PS), and a modified version of it, psNTP9-DM (DM), are expressed in <i>Saccharomyces cerevisiae</i>, they localize to nuclei, binding to largely non-overlapping promoter regions of chromatin. PS- and DM-expressing yeast also exhibit different expression profiles for potentially regulated target genes, consistent with observed phenotypes. In the present study, we use ChIP-seq assays to show that PS and DM also associate with largely different promoter regions of Arabidopsis genes, with similar non-overlapping expression profiles for potential target genes. Functional studies, using electrophoretic mobility shift assays (EMSA), verified PS-specific binding to yeast or plant promoter binding sites. DM binding to both heterologous dsDNA "],"journal":["Plants (Basel, Switzerland)"],"pubmed_title":["Chromatin-Associated Pea Apyrase psNTP9 Function as a DNA-Binding Regulatory Protein in Yeast and Arabidopsis."],"pmcid":["PMC12656381"],"funding_grant_id":["UTA13-000682"],"pubmed_authors":["Clark G","Roux SJ","Slocum RD","Wang H","Cai X"],"additional_accession":[]},"is_claimable":false,"name":"Chromatin-Associated Pea Apyrase psNTP9 Function as a DNA-Binding Regulatory Protein in Yeast and Arabidopsis.","description":"As reported in earlier work, when a pea apyrase, psNTP9 (PS), and a modified version of it, psNTP9-DM (DM), are expressed in <i>Saccharomyces cerevisiae</i>, they localize to nuclei, binding to largely non-overlapping promoter regions of chromatin. PS- and DM-expressing yeast also exhibit different expression profiles for potentially regulated target genes, consistent with observed phenotypes. In the present study, we use ChIP-seq assays to show that PS and DM also associate with largely different promoter regions of Arabidopsis genes, with similar non-overlapping expression profiles for potential target genes. Functional studies, using electrophoretic mobility shift assays (EMSA), verified PS-specific binding to yeast or plant promoter binding sites. DM binding to both heterologous dsDNA ","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Nov","modification":"2026-07-05T03:18:22.822Z","creation":"2026-07-05T03:12:28.097Z"},"accession":"S-EPMC12656381","cross_references":{"pubmed":["41304665"],"doi":["10.3390/plants14223514"]}}