biostudies-literatureTang PNatural Science Foundation of Jiangxi Province of ChinaNational Natural Science Foundation of China1087https://www.ebi.ac.uk/biostudies/studies/S-EPMC12656734biostudies-literatureUnknown12(11)While previous studies have extensively demonstrated that summer heat stress (HS) impairs oocyte quality via follicular granulosa cell (GC) mediation, the molecular mechanisms underlying HS-induced GC-mediated oocyte damage-particularly at the multi-omics level-remain poorly understood. This study integrated metabolomic and transcriptomic analyses of GCs from gilts under seasonal thermal stress (winter CON vs. summer HS) to elucidate GC-mediated regulatory networks affecting oocyte quality. Non-targeted metabolomics identified 45 differentially accumulated metabolites (DAMs, <i>p</i> < 0.05), with 69% being lipids/lipid-like molecules enriched in pathways such as glycerophospholipid metabolism, choline metabolism, linoleic acid metabolism, the adipocytokine signaling pathway, and the sphinVeterinary sciencesIntegrated Metabolomic and Transcriptomic Analysis Decodes Heat Stress-Induced Metabolic Shifts in Gilt Granulosa Cells.PMC126567343220276520224BAB215055Liu XXie XHuang JYin CTang PSi XShi YfalseIntegrated Metabolomic and Transcriptomic Analysis Decodes Heat Stress-Induced Metabolic Shifts in Gilt Granulosa Cells.While previous studies have extensively demonstrated that summer heat stress (HS) impairs oocyte quality via follicular granulosa cell (GC) mediation, the molecular mechanisms underlying HS-induced GC-mediated oocyte damage-particularly at the multi-omics level-remain poorly understood. This study integrated metabolomic and transcriptomic analyses of GCs from gilts under seasonal thermal stress (winter CON vs. summer HS) to elucidate GC-mediated regulatory networks affecting oocyte quality. Non-targeted metabolomics identified 45 differentially accumulated metabolites (DAMs, <i>p</i> < 0.05), with 69% being lipids/lipid-like molecules enriched in pathways such as glycerophospholipid metabolism, choline metabolism, linoleic acid metabolism, the adipocytokine signaling pathway, and the sphin2025-01-01T00:00:00Z2025 Nov2026-05-19T03:17:03.991Z2026-05-19T03:11:32.952ZS-EPMC126567344129572510.3390/vetsci12111087